Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan 701, Taiwan.
Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan 701, Taiwan.
Eur J Protistol. 2022 Feb;82:125860. doi: 10.1016/j.ejop.2021.125860. Epub 2021 Dec 17.
The current study investigated the potential functions of amoeboid cell formation and migration in a thraustochytrid strain, Aurantiochytrium limacinum BL10. Our results showed that: (1) When the surface of an agar plate was inoculated with BL10, amoeboid cells mainly emerged on the periphery of isolated colonies. The amoeboid cells then migrated outwards to form small vegetative cell clusters, which favored rapid colony expansion. In addition, amoeboid cells were capable of self-recognition (i.e. they were able to distinguish BL10 from other thraustochytrid species), and could choose whether to evade (self colonies) or approach (non-self colonies). These observations indicated that amoeboid cells were employed by BL10 to help colonize empty territories and to outcompete other thraustochytrid species in previously colonized territories. (2) When the agar medium was soft, amoeboid cells were able to penetrate the surface and migrate throughout, thereby allowing BL10 to colonize the interior of the solid matrix. This finding suggested that amoeboid cell formation and migration may help Aurantiochytrium colonize the interior of solid matrices to obtain additional nutrients and spatial resources. The mechanisms underlying the regulation of amoeboid cell formation and migration as well as the extraordinary microbial social behaviors of BL10 are also discussed in this article.
本研究探讨了变形细胞形成和迁移在甲藻菌株 Aurantiochytrium limacinum BL10 中的潜在功能。我们的结果表明：（1）当琼脂平板表面接种 BL10 时，变形细胞主要出现在孤立菌落的外周。然后，变形细胞向外迁移形成小的营养细胞簇，有利于快速的菌落扩张。此外，变形细胞能够自我识别（即能够区分 BL10 与其他甲藻），并可以选择逃避（自我菌落）或接近（非自我菌落）。这些观察表明，变形细胞被 BL10 用来帮助占领空旷的领地，并在已占领的领地中与其他甲藻竞争。（2）当琼脂培养基变软时，变形细胞能够穿透表面并在整个培养基中迁移，从而使 BL10 能够在固体基质的内部定殖。这一发现表明，变形细胞的形成和迁移可能有助于 Aurantiochytrium 定殖于固体基质的内部，以获得额外的营养和空间资源。本文还讨论了变形细胞形成和迁移的调控机制以及 BL10 非凡的微生物社会行为。
