Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Clinical Trial Center of Zhongnan Hospital, Wuhan University, Wuhan 430071, PR China.
Food Chem. 2022 May 30;377:131962. doi: 10.1016/j.foodchem.2021.131962. Epub 2021 Dec 29.
In this study, a highly sensitive method for analysis of 4 cyanogenic glycosides (CNGs) in cold-pressed flaxseed oil was developed by using cigarette filter fiber-based SPE and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The cold-pressed flaxseed oil was diluted with 5% (v/v) isopropanol/n-hexane solution and loaded to a cigarette filters fiber-based SPE column for CNG enrichment and purification. Under optimized conditions, four CNGs could be detected with limits of detection ranging from 1.3 to 4.4 pg/mL. The linear range was 0.05-50 ng/ml with a linear correlation coefficient (r) > 0.9935. CNG recovery ranged from 113% to 133%, and the relative standard deviation was between 0.8% and 20.5%. Finally, the proposed method was applied to the determination of CNGs in nine cold-pressed flaxseed oils.
本研究采用基于香烟过滤纤维的固相萃取（SPE）和超高效液相色谱-串联质谱法（UPLC-MS/MS），开发了一种用于分析冷榨亚麻籽油中 4 种生氰糖苷（CNGs）的高灵敏度方法。将冷榨亚麻籽油用 5%（v/v）异丙醇/正己烷溶液稀释，加载到基于香烟过滤纤维的 SPE 柱上，用于 CNG 的富集和纯化。在优化条件下，可检测到 4 种 CNGs，检出限范围为 1.3-4.4 pg/mL。线性范围为 0.05-50 ng/ml，线性相关系数（r）>0.9935。CNG 回收率为 113%-133%，相对标准偏差在 0.8%-20.5%之间。最后，该方法应用于测定 9 种冷榨亚麻籽油中的 CNGs。
