Asano Y, Shibuya T, Okamura S, Yamaga S, Otsuka T, Niho Y
First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Cancer Res. 1987 Nov 1;47(21):5647-8.
The effects of human recombinant granulocyte/macrophage colony-stimulating factor (GM-CSF) and human native purified granulocyte colony-stimulating factor (G-CSF) on the growth of clonogenic leukemic blast cells from eight Japanese patients with acute myeloblastic leukemia were studied, using an in vitro leukemic blast colony assay. The results showed that GM-CSF stimulated leukemic blast colony formation in all cases examined, whereas G-CSF stimulated colony formation in four of the eight cases. The maximum stimulating activity of GM-CSF on the growth of clonogenic leukemic blast cells was higher than that of G-CSF in the majority of cases, while sometimes GM-CSF and G-CSF worked synergistically. Thus, the clonogenic leukemic blast cell populations seemed to be heterogeneous with respect to their in vitro response to growth regulators.
采用体外白血病原始细胞集落测定法，研究了人重组粒细胞/巨噬细胞集落刺激因子（GM-CSF）和人天然纯化粒细胞集落刺激因子（G-CSF）对8例日本急性髓细胞白血病患者的克隆性白血病原始细胞生长的影响。结果显示，GM-CSF在所有检测病例中均刺激白血病原始细胞集落形成，而G-CSF在8例中的4例刺激集落形成。在大多数病例中，GM-CSF对克隆性白血病原始细胞生长的最大刺激活性高于G-CSF，而有时GM-CSF和G-CSF协同发挥作用。因此，克隆性白血病原始细胞群体在体外对生长调节因子的反应方面似乎是异质性的。
