Raknam Panithi, Balekar Neelam, Teanpaisan Rawee, Amnuaikit Thanaporn
Department of Pharmaceutical Technology and Drug Delivery System Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla, Thailand.
IPS Academy College of Pharmacy, Indore, India.
J Oral Microbiol. 2021 Dec 19;14(1):2012390. doi: 10.1080/20002297.2021.2012390. eCollection 2022.
SD11 is a probiotic derived from the human oral cavity and has potential being used for dental prophylaxis. The cell free supernatant (CFS) of SD11 has good antimicrobial and antioxidant effects.
This study aimed to incorporate CFS of the probiotic into thermoresponsive copolymers to create a sol-gel formulation.
The sol-gel formulation was developed using Poloxamer 407 as the main polymer, which was mixed with natural polymers such as gellan gum, sodium alginate, and xyloglucan in different proportions. The sol-gel formulations were characterized based on their physicochemical parameters such as appearance, pH, viscosity, flow-ability in low temperature, antioxidant and antibacterial activity. An release study was performed using Franz's diffusion cell and the stability was determined under freeze-thaw cycle conditions.
The combination of 15% w/v of poloxamer 407 with 0.5% w/v of sodium alginate was the best sol-gel formulation to deliver the CFS of the probiotic.
This study was successful in creating a sol-gel formulation using a thermoresponsive copolymer, that could efficiently deliver CFS of the probiotic SD11.
SD11是一种源自人类口腔的益生菌，具有用于牙齿预防的潜力。SD11的无细胞上清液（CFS）具有良好的抗菌和抗氧化作用。
本研究旨在将益生菌的CFS掺入热响应性共聚物中，以制备溶胶-凝胶制剂。
以泊洛沙姆407为主要聚合物，与结冷胶、海藻酸钠和木葡聚糖等天然聚合物按不同比例混合，制备溶胶-凝胶制剂。根据其外观、pH值、粘度、低温流动性、抗氧化和抗菌活性等理化参数对溶胶-凝胶制剂进行表征。使用弗兰兹扩散池进行释放研究，并在冻融循环条件下测定稳定性。
15%w/v的泊洛沙姆407与0.5%w/v的海藻酸钠组合是递送益生菌CFS的最佳溶胶-凝胶制剂。
本研究成功地使用热响应性共聚物制备了一种溶胶-凝胶制剂，该制剂能够有效地递送益生菌SD11的CFS。
