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利用新一代测序技术获得中国森林冠蜥(有鳞目,鬣蜥科)的完整线粒体基因组

The complete mitochondrial genome of the forest crested lizard, (Squamata, Agamidae) in China by the next generation sequencing.

作者信息

Ma Wenfang, Jing Xiaotong, Wei Xiaomei, Huang Yong

机构信息

Guangxi University of Chinese Medicine, Nanning, Guangxi, China.

Guangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi, China.

出版信息

Mitochondrial DNA B Resour. 2021 Dec 19;7(1):141-143. doi: 10.1080/23802359.2021.2008822. eCollection 2022.

Abstract

The whole mitogenome can prove useful tools for phylogenetic reconstruction and efficiently recover with reasonable taxon sampling. is widely distributed and arboreal in habits. However, studies of are still very limited, including population genetics and evolutionary biology. In this study, we reported the complete mitochondrial genome of the by next-generation sequencing for future more researches on systematics and evolution of from the perspective of mitochondrial DNA. The length of mitogenome was 17,688 bp, including 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 tRNA genes and a control region. The phylogenetic tree recovered the monophyly of the and revealed that newly sequenced well supported as the sister taxon to by very high posterior probabilities (1.0). The complete mitochondrial genome of in this study will be helpful for understanding the phylogenetic systematics and relationships, and molecular evolution of in Agamidae.

摘要

整个线粒体基因组可成为系统发育重建的有用工具,并通过合理的分类群采样有效地恢复。它分布广泛,习性为树栖。然而,对其的研究仍然非常有限,包括群体遗传学和进化生物学。在本研究中,我们通过下一代测序报道了该物种的完整线粒体基因组,以便未来从线粒体DNA的角度对其系统学和进化进行更多研究。线粒体基因组长度为17,688 bp,包括13个蛋白质编码基因(PCGs)、2个核糖体RNA(rRNA)基因、22个转运RNA(tRNA)基因和一个控制区。系统发育树恢复了该物种的单系性,并显示新测序的物种以非常高的后验概率(1.0)很好地支持作为另一物种的姐妹分类群。本研究中该物种的完整线粒体基因组将有助于理解鬣蜥科中该物种的系统发育系统学和关系以及分子进化。

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