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下一代测序产生了一个几乎完整的线粒体基因组,该基因组来自中国特有的(爬行纲,有鳞目,石龙子科)物种。

Next-generation sequencing yields a nearly complete mitochondrial genome of (Reptilia, Squamata, Scincidae) endemic to China.

作者信息

Wu Na, Cai Bo, Chen Minli, Guo Xianguang

机构信息

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.

University of Chinese Academy of Sciences, Beijing, China.

出版信息

Mitochondrial DNA B Resour. 2020 Nov 3;5(3):3655-3656. doi: 10.1080/23802359.2020.1832594.

Abstract

The Liu's skink, , is endemic to China. In this study, a nearly complete mitochondrial genome (mitogenome) (17,643 bp in length) of from Junzhang Mountain, Wuxi, Jiangsu province, China, was determined by next-generation sequencing. Similar to the typical mtDNA of vertebrates, it contained two ribosomal RNA genes, 13 protein-coding genes (PCGs), 22 transfer RNA genes, and one control region (CR). With exception to the CR, all of the 37 genes were completely recovered. The PCGs were used to perform Bayesian phylogenetic analyses together with other scincid lizards with mitogenome data in GenBank. The resulting phylogenetic tree supported the monophyly of the group, and suggested that is the sister taxon to plus . The mitogenome of will provide fundamental data for the exploration of the mitogenome evolution in skinks.

摘要

刘氏石龙子(Liu's skink)仅分布于中国。在本研究中,通过二代测序技术测定了来自中国江苏省无锡市军嶂山的刘氏石龙子的一个近乎完整的线粒体基因组(线粒体基因组全长17,643 bp)。与典型的脊椎动物线粒体DNA相似,它包含两个核糖体RNA基因、13个蛋白质编码基因(PCGs)、22个转运RNA基因和一个控制区(CR)。除控制区外,所有37个基因均被完整获得。利用这些蛋白质编码基因与GenBank中其他具有线粒体基因组数据的石龙子蜥蜴一起进行贝叶斯系统发育分析。得到的系统发育树支持刘氏石龙子类群的单系性,并表明刘氏石龙子是刘氏滑蜥(Scincella liuchengchaoi)与宁波滑蜥(Scincella modesta)组合的姐妹分类单元。刘氏石龙子的线粒体基因组将为探索石龙子线粒体基因组进化提供基础数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf5a/7646586/140b26ce51f9/TMDN_A_1832594_F0001_B.jpg

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