一种用于肿瘤相关mRNA成像的基于荧光共振能量转移的新型树枝状杂交链式反应。
A novel FRET-based dendritic hybridization chain reaction for tumour-related mRNA imaging.
作者信息
Liu Shiyuan, Liu Zhenxiang, Wang Huimin, Wang Jiaoli, Liu Ruiting, Wang Kemin, Huang Jin
机构信息
State Key Laboratory of Chemo/Biosensing and Chemometrics, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, China.
出版信息
Chem Commun (Camb). 2022 Jan 27;58(9):1414-1417. doi: 10.1039/d1cc06009k.
PMID:34994763
Abstract
A novel FRET-based dendritic hybridization chain reaction (D-HCR) for TK1 mRNA imaging in living cells was developed. Compared with traditional complex D-HCR methods, it includes the advantages of having a simple design, an accurate signal and is suitable for use with living cells.
摘要
开发了一种用于活细胞中胸苷激酶1（TK1）mRNA成像的基于荧光共振能量转移（FRET）的新型树枝状杂交链式反应（D-HCR）。与传统的复杂D-HCR方法相比，它具有设计简单、信号准确且适用于活细胞的优点。
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