一种用于肿瘤细胞中高灵敏度和高选择性mRNA成像的超级三明治荧光原位杂交策略。
A supersandwich fluorescence in situ hybridization strategy for highly sensitive and selective mRNA imaging in tumor cells.
作者信息
Huang Jin, Wang He, Yang Xiaohai, Yang Yanjing, Quan Ke, Ying Le, Xie Nuli, Ou Min, Wang Kemin
机构信息
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China.
出版信息
Chem Commun (Camb). 2016 Jan 7;52(2):370-3. doi: 10.1039/c5cc08503a. Epub 2015 Nov 2.
We report a supersandwich fluorescence in situ hybridization (SFISH) strategy for highly sensitive and selective in situ visualization of mRNA expression patterns at the single-cell level. This strategy uses two fluorophore-labeled signal probes to generate a supersandwich product, which in turn generates numerous signal probes located at the target mRNA position, resulting in the in situ fluorescence signal amplification.
我们报道了一种超三明治荧光原位杂交（SFISH）策略，用于在单细胞水平上对mRNA表达模式进行高灵敏度和高选择性的原位可视化。该策略使用两个荧光团标记的信号探针生成超三明治产物，该产物又会在目标mRNA位置产生大量信号探针，从而实现原位荧光信号放大。
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