Huang Jin, Wang He, Yang Xiaohai, Yang Yanjing, Quan Ke, Ying Le, Xie Nuli, Ou Min, Wang Kemin
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China.
Chem Commun (Camb). 2016 Jan 7;52(2):370-3. doi: 10.1039/c5cc08503a. Epub 2015 Nov 2.
We report a supersandwich fluorescence in situ hybridization (SFISH) strategy for highly sensitive and selective in situ visualization of mRNA expression patterns at the single-cell level. This strategy uses two fluorophore-labeled signal probes to generate a supersandwich product, which in turn generates numerous signal probes located at the target mRNA position, resulting in the in situ fluorescence signal amplification.
我们报道了一种超三明治荧光原位杂交（SFISH）策略，用于在单细胞水平上对mRNA表达模式进行高灵敏度和高选择性的原位可视化。该策略使用两个荧光团标记的信号探针生成超三明治产物，该产物又会在目标mRNA位置产生大量信号探针，从而实现原位荧光信号放大。
