Ford R J, Mehta S, Sharma S
Department of Molecular Pathology, University of Texas, M.D. Anderson Hospital and Tumor Institute, Houston 77030.
Leukemia. 1987 Apr;1(4):386-9.
Hairy cell leukemia cell lines were established from eight untreated patients using purified B cell growth factor (BCGF) in vitro. These cell lines maintained their original cell surface immunophenotype for about 1 month, after which they began to lose one or more of their characteristic surface antigens. The cell lines also maintained typical hairy cell leukemia morphology for 2-3 months in vitro but later showed an increasing number of multinucleate giant cells that maintained a B cell surface phenotype. The cell lines became independent of exogenously provided BCGF after at least 1 month in vitro and secreted BCGF activity into culture supernatants in most cases. Some cell lines also acquired Epstein-Barr virus nuclear antigen positivity after variable period. Two hairy cell leukemia patients also showed hyperactive T cell responses in vitro and exhibited spontaneous T cell proliferation in culture without exogenously supplied interleukin-2. These T cell lines had the T helper phenotype and secreted significant amounts of T cell-associated lymphokines with BCGF and interleukin-2 activity into culture supernatants.
利用纯化的B细胞生长因子（BCGF）在体外从8例未经治疗的患者中建立了毛细胞白血病细胞系。这些细胞系在约1个月内保持其原始细胞表面免疫表型，此后它们开始丢失一种或多种特征性表面抗原。这些细胞系在体外2 - 3个月内也保持典型的毛细胞白血病形态，但后来出现越来越多保持B细胞表面表型的多核巨细胞。这些细胞系在体外培养至少1个月后不再依赖外源性提供的BCGF，并且在大多数情况下将BCGF活性分泌到培养上清液中。一些细胞系在不同时期后也获得了爱泼斯坦 - 巴尔病毒核抗原阳性。两名毛细胞白血病患者在体外也表现出T细胞反应亢进，并且在没有外源性提供白细胞介素 - 2的情况下在培养中表现出自发性T细胞增殖。这些T细胞系具有T辅助细胞表型，并将大量具有BCGF和白细胞介素 - 2活性的T细胞相关淋巴因子分泌到培养上清液中。
