Engineering Lab for Water Pollution Control and Resources Recovery of Jilin Province, School of Environment, Northeast Normal University, Changchun, 130117, Jilin, China.
Engineering Lab for Water Pollution Control and Resources Recovery of Jilin Province, School of Environment, Northeast Normal University, Changchun, 130117, Jilin, China.
Environ Res. 2022 May 15;208:112639. doi: 10.1016/j.envres.2021.112639. Epub 2022 Jan 4.
Boron (B) industry and consuming produce large amounts of B-containing wastewater. Low tolerance of microorganisms and plants resulted in the biological removal of B was limited. Microalgae show high adaptability in adverse environments. Whether microalgae able to be utilized in B removal meanwhile produce bioresources, and the B tolerant mechanisms and regulation pathway of microalgae are unclear. In this study, the cell growth, B removal, and lipid/starch production of Chlorella regularis under different levels of B stress (0.5, 10, 25, and 50 mg/L) were examined. The mechanisms of signal perception and response were explored by transcriptome and network analysis. Microalgae tolerated 25 mg/L high B stress, cell growth showed no decline and biomass reach up to 4.5 g/L. Microalgae took in B with 3.35 mg/g and bonded them to protein and carbon components in cells, the B removal capability was higher than some special adsorbents. Microalgae produced 188.65 mg/(L∙d) lipids and 305.35 mg/(L∙d) starch. The mitogen-activated protein-kinase signaling pathway was involved in the B tolerance of microalgae and regulated B efflux, glycolysis, and lipid/starch accumulation to relieve B stress. This study provides potential biological technique for B removal in wastewater and promotes new insight into signal role in toxic pollutants biological treatment.
硼（B）工业和消费会产生大量含硼废水。微生物和植物对硼的耐受性低，导致生物去除硼的效果有限。微藻在恶劣环境中表现出很高的适应性。微藻是否能够用于去除硼并同时产生生物资源，以及微藻对硼的耐受机制和调节途径尚不清楚。本研究考察了不同硼胁迫水平（0.5、10、25 和 50mg/L）下小球藻的细胞生长、硼去除和脂质/淀粉生产。通过转录组和网络分析探讨了信号感知和响应的机制。微藻耐受 25mg/L 的高硼胁迫，细胞生长没有下降，生物量达到 4.5g/L。微藻吸收了 3.35mg/g 的硼，并将其结合到细胞的蛋白质和碳组分中，其硼去除能力高于一些特殊的吸附剂。微藻产生了 188.65mg/（L·d）的脂质和 305.35mg/（L·d）的淀粉。丝裂原活化蛋白激酶信号通路参与了微藻对硼的耐受，并调节硼外排、糖酵解和脂质/淀粉积累，以缓解硼胁迫。本研究为废水除硼提供了潜在的生物技术，并为信号在有毒污染物生物处理中的作用提供了新的见解。
