College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316000, China.
College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, China.
Bioresour Technol. 2019 Jun;282:118-124. doi: 10.1016/j.biortech.2019.03.011. Epub 2019 Mar 5.
This study investigated the biomass/lipid production, nutrient removal and fatty acid composition of an isolated mixotrophic microalga (Chlorella sp. G-9) cultured in simulated wastewater with different TOC/TN ratio. As the TOC/TN ratio of wastewater increased from 0 to 24, the growth rate of Chlorella sp. G-9 increased gradually, but did not increase further at 30. Nutrient removal was related to microalgae growth. In the wastewater with TOC/TN ratio of 24 and 30, 99.58% and 99.61% nitrogen was removed, respectively. In conditions of initial TOC/TN ratios of 24 and 30, Chlorella sp. G-9 could accumulate lipid as high as 35.3% and 36.5%, respectively. The corresponding lipid productivities were 34.2 and 32.6 mg L d, respectively, which were 13.7 and 13.0 times higher than those in photoautotrophic condition. Increasing the initial TOC/TN ratio of the wastewater could slightly increase the saturated degree in fatty acid, thereby improving the stability of biodiesel.
本研究考察了在不同 TOC/TN 比的模拟废水中培养的混养微藻（小球藻 G-9）的生物量/脂质生产、养分去除和脂肪酸组成。随着废水 TOC/TN 比从 0 增加到 24，小球藻 G-9 的生长速率逐渐增加，但在 30 时没有进一步增加。养分去除与微藻生长有关。在 TOC/TN 比为 24 和 30 的废水中，分别去除了 99.58%和 99.61%的氮。在初始 TOC/TN 比为 24 和 30 的条件下，小球藻 G-9 分别可以积累高达 35.3%和 36.5%的脂质。相应的脂质生产力分别为 34.2 和 32.6mg/L/d，分别比光自养条件下高 13.7 和 13.0 倍。增加废水的初始 TOC/TN 比可以略微增加脂肪酸的饱和度，从而提高生物柴油的稳定性。
