Risk Assessment Division, Environmental Health Research Department, National Institute of Environmental Research, Incheon 22689, Republic of Korea; Department of Environmental Health Science, Konkuk University, Seoul 05029, Republic of Korea.
Risk Assessment Division, Environmental Health Research Department, National Institute of Environmental Research, Incheon 22689, Republic of Korea.
Sci Total Environ. 2022 Apr 1;815:152846. doi: 10.1016/j.scitotenv.2021.152846. Epub 2022 Jan 5.
1,2,3-benzotriazole (BT) is used in large amounts around the world and is one of the substances derived from household chemicals that are of concern for risk when discharged to aquatic environments. Therefore, several studies have been conducted on the aquatic toxicity effects of BT, but the chronic impact assessment studies to evaluate the developmental effects on the early-life stage of fish are insufficient. In this study, the acute toxicity test and subchronic toxicity test (fish, early-life stage toxicity test, ELS test) using embryos of Japanese medaka (Oryzias latipes) were performed to evaluate the acute toxicity, developmental toxicity, growth (indicated by total length and weight at the end of the test), and histopathological effect of BT. In the short-term toxicity test on embryo and sac-fry stage, toxicity value was calculated to be 41 mg/L (NOEC). Based on this value, the exposure concentration of the ELS test was determined as 0.04, 0.4, 4 and 40 mg/L, and total exposure duration was 42 days. At the highest concentration group (40 mg/L), failure of swim bladder inflation and decrease of survival and size (total length and weight) were observed. Moreover, in the histopathological analysis, abnormal findings were detected in swim bladders from the 40 mg/L group such as inflammation and tumor changes. On the other hands, condition index (weight-length relationships, CI) was statistically significantly lower in all exposed groups compared to the control group. NOEC for the survival of BT was calculated to be 4 mg/L. LOEC for CI was 0.04 mg/L, which means BT inhibited weight gain relative to its length on larvae of medaka.
1,2,3-苯并三唑（BT）在世界范围内大量使用，是家庭化学品中对水生环境排放时具有风险的物质之一。因此，已经进行了多项关于 BT 的水生毒性效应的研究，但评估鱼类早期生命阶段发育影响的慢性影响评估研究还不够。在这项研究中，使用日本青鳉（Oryzias latipes）的胚胎进行了急性毒性试验和亚慢性毒性试验（鱼类，早期生命阶段毒性试验，ELS 试验），以评估 BT 的急性毒性、发育毒性、生长（以试验结束时的总长度和重量表示）和组织病理学效应。在胚胎和囊胚阶段的短期毒性试验中，计算出毒性值为 41mg/L（NOEC）。基于该值，ELS 试验的暴露浓度确定为 0.04、0.4、4 和 40mg/L，总暴露时间为 42 天。在最高浓度组（40mg/L）中，观察到鳔充气失败以及存活率和大小（总长度和重量）下降。此外，在组织病理学分析中，在 40mg/L 组的鳔中检测到异常发现，如炎症和肿瘤变化。另一方面，与对照组相比，所有暴露组的条件指数（体重-体长关系，CI）均显著降低。BT 对存活的 NOEC 计算为 4mg/L。CI 的 LOEC 为 0.04mg/L，这意味着 BT 抑制了青鳉幼虫的体重相对于其长度的增加。
