National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China; School of Food Science and Bioengineering, Changsha University of Science and Technology, Changsha, Hunan, PR China.
National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Poult Sci. 2022 Mar;101(3):101619. doi: 10.1016/j.psj.2021.101619. Epub 2021 Nov 29.
It's a difficult task for researchers to identify the gender of chicken eggs by nondestructive approach in the early of incubation, which not only could reduce the cost of incubation, but also could improve the welfare of chicks. Therefore, SPME/GC-MS has been applied to investigate its potential as a nondestructive tool for characterizing the differences of odor between male and female chicken eggs during early of incubation and even before hatch. The results showed that more volatiles were found in female White leghorn eggs during early of incubation and 6,10-dimethyl-5,9-undecadien-2-one, 6-methyl-5-hepten-2-one, nonanal, decanal, octanal, 2-nonen-1-ol, etc. were important for the distinction of male and female White leghorn eggs during E-E of incubation. 2-ethyl-1-hexanol; octanal, nonanal, 2,2,4-trimethyl-3-carboxyisopropyl pentanoic acid isobutyl ester; 2-nonen-1-ol, cyclopropanecarboxamide, heptadecane were correlated with gender of unhatched White leghorn, Hy-line brown and Jing fen eggs, respectively. Moreover, sex-related volatiles have been strongly influenced by incubation process and egg breed, and to be related to steroid hormone biosynthesis. What's more, this study enables us to develop a new visual for ovo sexing of chicken eggs and advances our understanding of the biological significance behind volatiles emitted from chicken eggs.
在孵化早期，研究人员很难通过非破坏性方法识别鸡蛋的性别，这不仅可以降低孵化成本，还可以提高小鸡的福利。因此，SPME/GC-MS 已被应用于研究其作为一种非破坏性工具的潜力，以在孵化早期甚至孵化前，对雄性和雌性鸡蛋之间的气味差异进行特征分析。结果表明，在孵化早期，更多的挥发性物质在雌性白来航鸡蛋中被发现，而 6,10-二甲基-5,9-十一碳二烯-2-酮、6-甲基-5-庚烯-2-酮、壬醛、癸醛、辛醛、2-壬烯-1-醇等物质对区分白来航鸡蛋的雌雄至关重要。2-乙基-1-己醇、辛醛、壬醛、2,2,4-三甲基-3-羧基异戊酸异丁酯、2-壬烯-1-醇、环丙烷甲酰胺、十七烷分别与未孵化的白来航、海兰褐和京粉鸡蛋的性别有关。此外，性别相关的挥发性物质受到孵化过程和鸡蛋品种的强烈影响，并与甾体激素生物合成有关。更重要的是，这项研究使我们能够开发一种新的鸡蛋性别鉴定方法，并加深我们对鸡蛋散发的挥发性物质背后的生物学意义的理解。
