Xing Yuqian, Liu Xiao, Pu Qinqin, Wu Min, Zhao Julia Xiaojun
Department of Chemistry, University of North Dakota, Grand Forks, North Dakota 58202, United States.
Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, North Dakota 58202, United States.
ACS Appl Bio Mater. 2018 Oct 15;1(4):1019-1027. doi: 10.1021/acsabm.8b00211. Epub 2018 Sep 21.
The G-quadruplex/hemin (G/H) complex has been broadly used in the field of bioanalytical chemistry for peroxidase-mimicking applications. The property of the G/H complex makes it possible to catalyze the decomposition of HO. The hydroxyl radical (·OH) generated during the procedure has a higher antibacterial performance than the original HO. Herein, an efficient and biocompatible antibacterial system, which provides the same antibacterial efficiency at lower HO concentration to alleviate the HO toxicity, has been demonstrated based on the conversion of HO to ·OH. With the G/H complex as the additive, the antibacterial activity of HO was vastly enhanced against both Gram-positive and Gram-negative bacteria in experiments. Furthermore, the designed antibacterial system was applied on the mice wound model and showed outstanding antibacterial activity of the G/H complex to prevent wound infection and facilitate wound healing. Our study renders the possibility of using the G/H complex to help control both Gram-positive and Gram-negative infections.
G-四链体/血红素（G/H）复合物已广泛应用于生物分析化学领域的过氧化物酶模拟应用中。G/H复合物的特性使其能够催化HO的分解。该过程中产生的羟基自由基（·OH）比原始的HO具有更高的抗菌性能。在此，基于HO向·OH的转化，已证明了一种高效且生物相容的抗菌系统，该系统在较低的HO浓度下提供相同的抗菌效率以减轻HO的毒性。以G/H复合物为添加剂，在实验中HO对革兰氏阳性菌和革兰氏阴性菌的抗菌活性均大大增强。此外，所设计的抗菌系统应用于小鼠伤口模型，显示出G/H复合物具有出色的抗菌活性，可预防伤口感染并促进伤口愈合。我们的研究使得使用G/H复合物来帮助控制革兰氏阳性菌和革兰氏阴性菌感染成为可能。
