Entomology and Nematology Department, Citrus Research and Education Center/IFAS, University of Florida, Lake Alfred, Florida, 33850, USA.
Sci Rep. 2022 Jan 7;12(1):125. doi: 10.1038/s41598-021-03184-0.
Wolbachia, a gram-negative [Formula: see text]-proteobacterium, is an endosymbiont found in some arthropods and nematodes. Diaphorina citri Kuwayama, the vector of 'Candidatus Liberibacter asiaticus' (CLas), are naturally infected with a strain of Wolbachia (wDi), which has been shown to colocalize with the bacteria pathogens CLas, the pathogen associated with huanglongbing (HLB) disease of citrus. The relationship between wDi and CLas is poorly understood in part because the complete genome of wDi has not been available. Using high-quality long-read PacBio circular consensus sequences, we present the largest complete circular wDi genome among supergroup-B members. The assembled circular chromosome is 1.52 megabases with 95.7% genome completeness with contamination of 1.45%, as assessed by checkM. We identified Insertion Sequences (ISs) and prophage genes scattered throughout the genomes. The proteins were annotated using Pfam, eggNOG, and COG that assigned unique domains and functions. The wDi genome was compared with previously sequenced Wolbachia genomes using pangenome and phylogenetic analyses. The availability of a complete circular chromosome of wDi will facilitate understanding of its role within the insect vector, which may assist in developing tools for disease management. This information also provides a baseline for understanding phylogenetic relationships among Wolbachia of other insect vectors.
沃尔巴克氏体（Wolbachia）是一种革兰氏阴性[Formula: see text]-变形菌，是一些节肢动物和线虫中的内共生体。柑橘木虱（Diaphorina citri Kuwayama）是‘亚洲韧皮杆菌候选种（CLas）’的载体，其体内自然感染了一种沃尔巴克氏体（wDi）菌株，该菌株已被证明与 CLas 细菌病原体共定位，CLas 是与黄龙病（HLB）相关的病原体。wDi 与 CLas 之间的关系尚未完全清楚，部分原因是 wDi 的完整基因组尚未可知。我们使用高质量的长读 PacBio 环形一致序列，呈现了超级群-B 成员中最大的完整环形 wDi 基因组。组装的环形染色体长 1.52 兆碱基，基因组完整性为 95.7%，污染率为 1.45%，这是通过 checkM 评估得出的。我们在基因组中发现了散布的插入序列（IS）和噬菌体基因。使用 Pfam、eggNOG 和 COG 对蛋白质进行注释，赋予了独特的结构域和功能。通过泛基因组和系统发育分析，将 wDi 基因组与先前测序的沃尔巴克氏体基因组进行了比较。wDi 完整环形染色体的可用性将有助于了解其在昆虫载体中的作用，这可能有助于开发疾病管理工具。该信息还为了解其他昆虫载体的沃尔巴克氏体之间的系统发育关系提供了基础。
