Wang Jinhu, Chen Junyu, Zhang Dongdong, Cui Xiaomei, Zhou Jinna, Li Jing, Wei Yanli, Bu Duo
College of Ecological Environment, Tibet University, 10 East Zangda Road, Lhasa 850000, China.
Hunan Eco-Geological Survey and Monitoring Institute, 256 Shaoshan North Road, Yuhua District, Changsha 410000, China.
Metabolites. 2023 Mar 6;13(3):388. doi: 10.3390/metabo13030388.
Microalgae are now widely applied in municipal (YH_3) and industrial sewage (YH_4) treatments. Through integrated omics analysis, we studied the similarities and differences at the molecular level between the two different types of sewage treatment processes. The most significantly enriched gene ontology (GO) terms in both types of sewage treatments were the ribosome, photosynthesis, and proteasome pathways. The results show that the pathways of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) were enriched for photosynthesis, glyoxylate and dicarboxylate metabolism, and carbon fixation in photosynthetic organisms. Considering YH_3 vs. YH_4, the metabolism of citrate, sedoheptulose-7P, and succinate was significantly upregulated. In addition, the results showed that the pathways of DEGs and DAMs were enriched in terms of amino acid metabolism and carotenoid biosynthesis in YH_4 vs. YH_3. The metabolism of S-Adenosyl-L-homocysteine was significantly downregulated, 2-oxobutanoate was significantly upregulated and downregulated, and the metabolism of abscisic acid glucose ester (ABA-GE) was also significantly upregulated. Overall, the results of this paper will help to improve the basic knowledge of the molecular response of microalgae to sewage treatments, and help design a response strategy based on microalgae for complex, mixed sewage treatments.
微藻目前广泛应用于城市污水（YH_3）和工业污水处理（YH_4）。通过综合组学分析，我们研究了两种不同类型污水处理过程在分子水平上的异同。两种污水处理中最显著富集的基因本体（GO）术语是核糖体、光合作用和蛋白酶体途径。结果表明，差异表达基因（DEG）和差异积累代谢物（DAM）的途径在光合作用、乙醛酸和二羧酸代谢以及光合生物中的碳固定方面得到了富集。考虑YH_3与YH_4，柠檬酸、景天庚酮糖-7P和琥珀酸的代谢显著上调。此外，结果表明，在YH_4与YH_3的比较中，DEG和DAM的途径在氨基酸代谢和类胡萝卜素生物合成方面得到了富集。S-腺苷-L-高半胱氨酸的代谢显著下调，2-氧代丁酸显著上调和下调，脱落酸葡萄糖酯（ABA-GE）的代谢也显著上调。总体而言，本文的结果将有助于提高微藻对污水处理分子响应的基础知识，并有助于设计基于微藻的复杂混合污水处理响应策略。
