Thikham Sudarat, Tongdonyod Sitthidat, Kantala Chatchawan, Therdtatha Phatthanaphong, Klangpetch Wannaporn
Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, 50100 Thailand.
Research Unit of Applied Electric Field in Engineering (RUEE), College of Integrated Science and Technology, Rajamangala University of Technology Lanna, Chiang Mai, 50220 Thailand.
J Food Sci Technol. 2024 Feb;61(2):320-330. doi: 10.1007/s13197-023-05843-8. Epub 2023 Sep 29.
Orange juice by-products, including peel, segments, and seeds, account for more than 50% of the total mass. This study aims to valorize the peel and segments of Sai Nam Phueng ( Blanco 'Sai Nam Phueng') orange juice by-products by producing crude pectic oligosaccharides (POS) with prebiotic potential using pulsed electric field (PEF)-assisted enzymatic treatment. PEF was performed for 5 min at field strengths of 10 and 7.5 kV/cm for orange peel powder (OPP) and orange segment powder (OSP), respectively, combined with Cellulase XL-531 at a concentration of 1.75%, pH 5.5, 40 °C for 2 h. The crude OPP-POS and OSP-POS yields were 19.16% and 17.51%, respectively, significantly higher than values obtained with PEF or enzymic hydrolysis singly. Thin layer chromatography results showed that the crude POS consisted of oligogalacturonic acids with various degrees of polymerization. Both POS products could enhance the growth of target probiotic bacteria and simultaneously produce short-chain fatty acids, especially propionic acid. Furthermore, the crude POS products also showed more than 90% resistance to simulated gastrointestinal digestion. These findings support the utilization of by-products from Sai Nam Phueng orange juice as a potential source for prebiotic production using PEF.
The online version contains supplementary material available at 10.1007/s13197-023-05843-8.
橙汁副产品，包括果皮、果瓣和果籽，占总质量的50%以上。本研究旨在通过脉冲电场（PEF）辅助酶处理生产具有益生元潜力的粗制果胶寡糖（POS），从而实现对赛南蓬（“赛南蓬”白肋脐橙）橙汁副产品的果皮和果瓣的增值利用。分别对橙皮粉（OPP）和橙瓣粉（OSP）在10 kV/cm和7.5 kV/cm的场强下进行5分钟的PEF处理，并与浓度为1.75%的纤维素酶XL - 531在pH 5.5、40℃下联合处理2小时。粗制OPP - POS和OSP - POS的产率分别为19.16%和17.51%，显著高于单独使用PEF或酶水解所获得的值。薄层色谱结果表明，粗制POS由不同聚合度的低聚半乳糖醛酸组成。两种POS产品都能促进目标益生菌的生长，同时产生短链脂肪酸，尤其是丙酸。此外，粗制POS产品对模拟胃肠消化的耐受性也超过90%。这些发现支持将赛南蓬橙汁副产品作为使用PEF生产益生元的潜在来源。
在线版本包含可在10.1007/s13197 - 023 - 05843 - 8获取的补充材料。
