Department of Surgery, S.H. Ho Urology Centre, Prince of Wales Hospital, The Chinese University of Hong Kong, 4/F LCW Clinical Sciences Building, 30-32 Ngan Shing Street, Shatin, New Territories, Hong Kong, China.
Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Hong Kong, China.
Sci Rep. 2022 Jan 7;12(1):22. doi: 10.1038/s41598-021-04035-8.
Urinary bladder cancer is a common cancer worldwide. Currently, the modality of treating and monitoring bladder cancer is wide. Nonetheless, the high recurrence rate of non-muscle-invasive bladder cancer after surgical resection is still unsatisfactory. Hereby, our study demonstrated whether the intra-operative and post-operative environments will affect bladder cancer recurrence utilizing in vitro cell line model. Bladder cancer cell lines were submerged in four different irrigating fluids for assessing their tumorigenic properties. Our results showed that sterile water performed the best in terms of the magnitude of cytotoxicity to cell lines. Besides, we also investigated cytotoxic effects of the four irrigating agents as well as mitomycin C (MMC) in normothermic and hyperthermic conditions. We observed that sterile water and MMC had an increased cytotoxic effect to bladder cancer cell lines in hyperthermic conditions. Altogether, our results could be translated into clinical practice in the future by manipulating the intra-operative and post-operative conditions in order to lower the chance of residual cancer cells reimplant onto the bladder, which in turns, reducing the recurrence rate of bladder cancers.
膀胱癌是一种常见的全球癌症。目前，膀胱癌的治疗和监测方式多种多样。然而，手术后非肌肉浸润性膀胱癌的高复发率仍然不尽如人意。因此，我们的研究利用体外细胞系模型证明了手术过程中和手术后的环境是否会影响膀胱癌的复发。膀胱癌细胞系被浸入四种不同的灌洗液中，以评估它们的致瘤特性。我们的结果表明，无菌水在细胞系的细胞毒性大小方面表现最佳。此外，我们还研究了四种灌洗液以及丝裂霉素 C（MMC）在常温与高温条件下的细胞毒性作用。我们观察到，在高温条件下，无菌水和 MMC 对膀胱癌细胞系的细胞毒性作用增强。总之，我们的研究结果可以通过操纵手术过程中和手术后的条件转化为临床实践，以降低残留癌细胞重新植入膀胱的机会，从而降低膀胱癌的复发率。
