State Key Laboratory of Eco-hydraulics in Northwest Arid Region of China, Xi'an University of Technology, Xi'an, 710048, China.
XianYang and Research Institute of Water Conservancy and Hydropower Planning and Design, XianYang, 712021, China.
Environ Sci Pollut Res Int. 2022 Apr;29(20):30265-30276. doi: 10.1007/s11356-021-17797-5. Epub 2022 Jan 8.
The new sugar source manno-oligosaccharide can regulate the structure of the microbial community. This study investigated the effects of adding manno-oligosaccharide at four different levels (0, 0.1%, 0.5%, and 1% w/w compost) to composting cow manure and straw on lignocellulose degradation and the bacterial community. Adding 0.5% manno-oligosaccharide had the greatest effects on accelerating the composting process, reducing its toxicity, and improving the stability of the product. After composting for 25 days, adding 0.5% manno-oligosaccharide decreased the hemicellulose, cellulose, and lignin contents to 2.25%, 11.25%, and 7.07%, respectively, compared with those under CK. Manno-oligosaccharide promoted the degradation of lignocellulose by increasing the abundances of Thermobifida, Streptomyces, and Luteimonas. In addition, manno-oligosaccharide inhibited pathogenic bacteria and increased the abundances of functional genes related to metabolism. Finally, adding 0.5% manno-oligosaccharide mainly affected the degradation of lignocellulose by enhancing the C/N ratio and the abundances of Streptomyces and the secretion system during composting according to redundancy analysis.
新型糖源甘露寡糖可调节微生物群落结构。本研究探讨了在堆肥牛粪和稻草中添加 4 种不同水平（0、0.1%、0.5%和 1%w/w 堆肥）甘露寡糖对木质纤维素降解和细菌群落的影响。添加 0.5%甘露寡糖对加速堆肥过程、降低毒性和提高产品稳定性的效果最大。在堆肥 25 天后，与 CK 相比，添加 0.5%甘露寡糖可将半纤维素、纤维素和木质素的含量分别降低至 2.25%、11.25%和 7.07%。甘露寡糖通过增加嗜热纤维单胞菌、链霉菌和路邓葡萄球菌的丰度来促进木质纤维素的降解。此外，甘露寡糖抑制了病原菌，并增加了与代谢相关的功能基因的丰度。最后，冗余分析表明，添加 0.5%甘露寡糖主要通过增强 C/N 比和链霉菌的丰度以及堆肥过程中的分泌系统来影响木质纤维素的降解。
