一步法和序贯 SARSCOV-2 聚合酶链反应检测并非每次都有效。
One-step and sequential SARSCOV-2 polymerase chain reaction tests would not work every time.
机构信息
Department of Infectious Diseases and Tropical Medicine, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
J Clin Lab Anal. 2022 Feb;36(2):e24226. doi: 10.1002/jcla.24226. Epub 2022 Jan 8.
INTRODUCTION
RT-PCR is widely used as a diagnostic test for the detection of SARS-CoV-2. In this study, we aim to describe the clinical utility of serial PCR testing in the final detection of COVID-19.
METHOD
We collected multiple nasopharyngeal swab samples from patients who had negative RT-PCR test on the first day after hospitalization. RT-PCR tests were performed on the second day for all patients with initial negative result. For the patients with secondary negative results on day 2, tertiary RT-PCR tests were performed on day 3 after hospitalization.
RESULT
Among 68 patients with initial negative test results, at the end of follow-up, the mortality number was 20 (29.4%). About 33.8% of patients had subsequent positive PCR test results for the second time and 17.4% of the patients who performed third PCR test had positive result.
CONCLUSION
Based on this study, serial RT-PCR testing is unlikely to yield additional information.
简介
实时逆转录聚合酶链反应（RT-PCR）被广泛用作检测 SARS-CoV-2 的诊断测试。本研究旨在描述在最终检测 COVID-19 时，连续进行 PCR 检测的临床应用。
方法
我们收集了住院后第一天 RT-PCR 检测结果为阴性的患者的多个鼻咽拭子样本。所有初次检测结果为阴性的患者均在第二天进行 RT-PCR 检测。对于第二次检测结果为阴性的患者，在住院后第三天进行第三次 RT-PCR 检测。
结果
在 68 例初始检测结果为阴性的患者中，随访结束时，死亡人数为 20 例（29.4%）。约 33.8%的患者第二次 PCR 检测结果为阳性，17.4%进行第三次 PCR 检测的患者结果为阳性。
结论
基于本研究，连续 RT-PCR 检测不太可能提供额外的信息。
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