Neobacillus paridis sp. nov., an endophyte of Paris polyphylla Smith var. yunnanensis.

A novel endophytic strain, designated YIM B02564, was isolated from the root of Paris polyphylla Smith var. yunnanensis obtained from Yunnan Province, southwest China. By using a polyphasic approach, cells of the strain were characterized as facultative anaerobic, Gram-positive and rod-shaped. The growth conditions of the strain were found to occur at 20-55 °C (optimum, 30 °C), pH 6.0-9.0 (optimum, pH 7.0). Strain YIM B02564 can tolerate 2% NaCl concentration. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM B02564 belonged to the genus Neobacillus and the 16S rRNA gene sequence similarity values of strain YIM B02564 to the type strains of members of this genus ranged from 95.6 to 97.8%. The DNA G+C content of strain YIM B02564 calculated from the whole genome sequence was 41.6 mol%. Values of the ANI and the dDDH between strain YIM B02564 and its closely related Neobacillus species were below 77.9% and 21.5%. Strain YIM B02564 contained MK-7 as the major menaquinone, iso-C and anteiso-C as the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid and four unidentified lipids. It contained meso-diaminopimelic acid in the cell-wall peptidoglycan. On the basis of polyphasic analysis, strain YIM B02564 could be differentiated genotypically and phenotypically from recognized species of the genus Neobacillus. The isolate therefore represents a novel species, for which the name Neobacillus paridis is proposed. The type strain is YIM B02564 (= JCM 34668 = CGMCC 1.18655).