Chryseobacterium paridis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis.

A Gram-negative, yellow-pigmented, rod-shaped bacterial strain YIM B02567 was isolated from the root of Paris polyphylla Smith var. yunnanensis in China. Strain YIM B02567 grew optimally at 25-30 °C and at pH 7.0 in the absence of NaCl on nutrient agar. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain YIM B02567 belong to the genus Chryseobacterium, and was closely related to Chryseobacterium piperi CTM and Chryseobacterium soli DSM 19298. Whole genome sequencing indicated that the genome size was 4,774,612 bp and with a G + C content of 34.5 mol%. Values of the ANI and the dDDH between strain YIM B02567 and its closely related Chryseobacterium species were below 81.72% and 24.7%. Strain YIM B02567 contained menaquinone-6 as the sole isoprenoid quinone, anteiso-C iso-C ω9c and iso-C 3-OH as major fatty acids and phosphatidylethanolamine as major polar lipid. Based on the polyphasic analyses, strain YIM B02567 could be differentiated genotypically and phenotypically from recognized species of the genus Chryseobacterium. The isolate, therefore, represents a novel species, for which the name Chryseobacterium paridis sp. nov. is proposed. The type strain is YIM B02567 (= CGMCC 1.18657).