Khatkhatay M I, Sankolli G M, Meherji P K, Gogate J, Chowdhury V, Joshi U M
Institute for Research in Reproduction (ICMR), Bombay, India.
Endocrinol Jpn. 1987 Aug;34(4):465-72. doi: 10.1507/endocrj1954.34.465.
A penicillinase linked enzyme immunoassay was developed for the estimation of pregnanediol-3 alpha-glucuronide (PdG) in urine. The immunoassay satisfied all the validity criteria and was used in detecting ovulation and in the assessment of corpus luteal function (CLF) during spontaneous or induced cycles. Reference values were established by estimating PdG levels in daily early morning urine samples during 31 menstrual cycles obtained from 17 regularly menstruating women. A PdG value of 1.7 micrograms/mg creatinine (micrograms/mgC) (90th Centile of follicular phase) in any MLP (mid-luteal phase) sample was considered as indicating ovulation. A value of 4.6 micrograms/mgC (20th centile of MLP) was considered to be evidence of sufficient CLF. When this approach was applied to 20 infertile cases, detection of the occurrence of ovulation/anovulation was made correctly in 19 out of 20 cases (95%). Accuracy was poor (55.6%) when the aim of the diagnosis was corpus luteal deficiency. Higher accuracy (88.9%) for corpus luteal deficiency/corpus luteal adequacy was obtained when the sum of PdG concentrations in three MLP samples were taken into consideration. A total of 13.8 micrograms/mgC (thrice the 20th centile for MLP) indicated probable corpus luteal deficiency, and values above this limit were considered to indicate corpus luteal adequacy.
开发了一种青霉素酶联酶免疫测定法用于测定尿中孕二醇-3α-葡萄糖醛酸苷（PdG）。该免疫测定法满足所有有效性标准，并用于检测排卵以及评估自然周期或诱导周期中的黄体功能（CLF）。通过估计17名月经规律女性的31个月经周期中每日清晨尿样中的PdG水平来确定参考值。任何黄体中期（MLP）样本中PdG值为1.7微克/毫克肌酐（微克/毫克C）（卵泡期第90百分位数）被认为表明排卵。4.6微克/毫克C（MLP第20百分位数）的值被认为是黄体功能充足的证据。当将这种方法应用于20例不育病例时，20例中有19例（95%）正确检测到排卵/无排卵的发生。当诊断目的是黄体功能不足时，准确性较差（55.6%）。当考虑三个MLP样本中PdG浓度的总和时，黄体功能不足/黄体功能充足的准确性更高（88.9%）。总共13.8微克/毫克C（MLP第20百分位数的三倍）表明可能存在黄体功能不足，高于此限值的值被认为表明黄体功能充足。
