Ladner M B, Martin G A, Noble J A, Nikoloff D M, Tal R, Kawasaki E S, White T J
Department of Molecular Biology, Cetus Corporation, Emeryville, CA 94608.
EMBO J. 1987 Sep;6(9):2693-8. doi: 10.1002/j.1460-2075.1987.tb02561.x.
Bone marrow progenitor cells differentiate into mononuclear phagocytes in the presence of colony stimulating factor-1 (CSF-1). Characterization of the human CSF-1 gene shows that it contains 10 exons and 9 introns, which span 20 kb. Analysis of multiple CSF-1 transcripts demonstrates that alternate use of exon 6 splice acceptor sites and 3' noncoding sequence exons occurs. These alternatively spliced transcripts can encode either a 224 or a 522 amino acid CSF-1. Implications of differential splicing for the production and function of CSF-1 are discussed.
在集落刺激因子-1（CSF-1）存在的情况下，骨髓祖细胞可分化为单核吞噬细胞。对人类CSF-1基因的特征分析表明，它包含10个外显子和9个内含子，跨度为20kb。对多个CSF-1转录本的分析表明，存在外显子6剪接受体位点和3'非编码序列外显子的交替使用情况。这些选择性剪接的转录本可以编码224个或522个氨基酸的CSF-1。文中讨论了差异剪接对CSF-1产生和功能的影响。
