氯喹处理细胞的高效多瘤病毒DNA转染

Luthman H, Magnusson G

Nucleic Acids Res. 1983 Mar 11;11(5):1295-308. doi: 10.1093/nar/11.5.1295.

Chloroquine treatment of rodent cells during the first hours of polyoma DNA transfection increase the fraction of cells expressing viral functions. The effect has been observed after DNA absorption using both the DEAE-dextran and calcium phosphate coprecipitation methods. Exposure to chloroquine increased the proportion of transfected mouse cells to approximately 40%. From a culture of one million such cells, microgram quantities of newly synthesized viral DNA could be isolated. Similarly, the transformation frequency of rat cells following polyoma DNA transfection was approximately 6-fold increased by chloroquine treatment. The effect of the compound was even more pronounced in transfections with linear forms of polyoma DNA, suggesting that chloroquine inhibits degradation of DNA absorbed by the cells.

在多瘤病毒DNA转染的最初几个小时内，用氯喹处理啮齿动物细胞可增加表达病毒功能的细胞比例。使用DEAE-葡聚糖和磷酸钙共沉淀法吸收DNA后均观察到了这种效果。暴露于氯喹可使转染的小鼠细胞比例增加到约40%。从一百万个这样的细胞培养物中，可以分离出微克量的新合成病毒DNA。同样，氯喹处理使多瘤病毒DNA转染后的大鼠细胞转化频率提高了约6倍。该化合物在用多瘤病毒DNA线性形式进行的转染中效果更为明显，这表明氯喹可抑制细胞吸收的DNA的降解。