Gordon J, Millsum M J, Guy G R, Ledbetter J A
Department of Immunology, University of Birmingham, GB.
Eur J Immunol. 1987 Oct;17(10):1535-8. doi: 10.1002/eji.1830171026.
Highly purified resting B lymphocytes stimulated for 3 days to high-rate DNA synthesis by a synergistic combination of phorbol dibutyrate and ionomycin soon returned to quiescence once those signals had been removed. The maintenance of DNA synthesis in such cultures was found to provide a sensitive assay for revealing the factors that interact with cycling B cells. Whereas several activities-namely, interleukin 4, anti-Bp50 and a low molecular weight B cell growth factor-were, by themselves, capable of prolonging DNA synthesis over a further day or so, no single factor was capable of sustaining the replication cycle out to day 6 of culture. By contrast, certain combinations of activities displayed significant synergy in the restimulation assay. The most striking observed was that between interleukin 4 and anti-Bp50 where, by day 6, their combined effect on maintaining DNA synthesis in 3-day stimulated cells was the same as having kept phorbol dibutyrate and ionomycin in the culture system. The implications of these findings are discussed.
高度纯化的静止B淋巴细胞，通过佛波酯二丁酯和离子霉素的协同组合刺激3天以进行高速率DNA合成，一旦去除这些信号，它们很快就会恢复静止状态。发现在此类培养物中维持DNA合成可提供一种灵敏的检测方法，以揭示与循环B细胞相互作用的因子。虽然几种活性物质，即白细胞介素4、抗Bp50和一种低分子量B细胞生长因子，自身能够将DNA合成延长大约一天左右，但没有单一因子能够将复制周期维持到培养的第6天。相比之下，某些活性物质的组合在再刺激检测中显示出显著的协同作用。观察到的最显著的协同作用是在白细胞介素4和抗Bp50之间，到第6天时，它们对维持3天刺激细胞中DNA合成的联合作用与在培养系统中保留佛波酯二丁酯和离子霉素的效果相同。讨论了这些发现的意义。
