School of Pharmacy and Health Sciences, Fairleigh Dickinson University, Florham Park, New Jersey, USA.
Biomed Chromatogr. 2022 Apr;36(4):e5332. doi: 10.1002/bmc.5332. Epub 2022 Feb 3.
Various polar stationary phases are available for hydrophilic interaction chromatography (HILIC) and help drive continuous applications in biomedical, environmental, and pharmaceutical areas in the past decade. Although the stationary phases for HILIC have been reviewed previously, it is an appropriate time to take another look at the progresses made during the past 5 years. The current review provides an overview of the polar stationary phases commercially available for HILIC applications in an effort to assist scientists in selecting suitable columns. New types of stationary phases that were published in literature in the past 5 years are summarized and discussed. The trend in stationary phase research and development is also highlighted. Of particular interest is the experimental evidence for direct interactions of polar analytes with the ligands of the stationary phases under HILIC conditions. In addition, two different approaches have been developed to delineate the relative significance of the partitioning and adsorption mechanisms in HILIC, representing an important advancement in our understanding of the retention mechanisms in HILIC.
各种极性固定相可用于亲水相互作用色谱（HILIC），并在过去十年中有助于推动生物医学、环境和制药领域的连续应用。尽管之前已经对 HILIC 的固定相进行了综述，但现在是时候回顾一下过去 5 年取得的进展了。本综述概述了商业上可用于 HILIC 应用的极性固定相，旨在帮助科学家选择合适的柱子。总结并讨论了过去 5 年内文献中发表的新型固定相。还强调了固定相研究和开发的趋势。特别感兴趣的是在 HILIC 条件下，极性分析物与固定相配体直接相互作用的实验证据。此外，已经开发出两种不同的方法来描绘 HILIC 中分配和吸附机制的相对重要性，这代表了我们对 HILIC 保留机制理解的重要进展。
