Yang J, Balakrishnan A, Hamamoto S, Elias J J, Rosenau W, Beattie C W, Das Gupta T K, Wellings S R, Nandi S
Cancer Research Laboratory, University of California, Berkeley 94720.
J Cell Physiol. 1987 Nov;133(2):228-34, 254-5. doi: 10.1002/jcp.1041330205.
Human breast epithelial cells derived from various sources (fibroadenoma, reduction mammoplasty, and mastectomy tissues from premenopausal patients) have been cultured in collagen gel matrix using serum-free medium. Response to various additives has been analyzed for growth-promoting effect when added to a basal medium containing insulin, cholera toxin, and BSA. A consistent observation has been the effect of EGF and cortisol in growth stimulation of human breast epithelial cells, while separately, each additive elicited only a small response. Under this condition, employing EGF and cortisol combinations, these cells gave rise to organized colonies consisting of clusters of cells, usually spherical, without any duct-like extensions. Ultrastructural and immunocytochemical studies, using a panel of monoclonal and polyclonal antibodies, have shown that cell types and features that can be identified in the original breast tissue can also be delineated in the progeny populations. The topographical feature, consisting of lumina surrounded by a single inner layer of epithelial cells and an outer layer of basal/myoepithelial cells, can be re-created in the collagen gel system starting from small clumps of cells.
源自各种来源（纤维腺瘤、缩乳术以及绝经前患者的乳房切除术组织）的人乳腺上皮细胞已在胶原凝胶基质中使用无血清培养基进行培养。当将各种添加剂添加到含有胰岛素、霍乱毒素和牛血清白蛋白的基础培养基中时，已对其促进生长的效果进行了分析。一个一致的观察结果是，表皮生长因子（EGF）和皮质醇对人乳腺上皮细胞的生长具有刺激作用，而单独使用时，每种添加剂仅引起微小反应。在这种条件下，使用EGF和皮质醇的组合，这些细胞形成了由细胞簇组成的有组织的集落，这些细胞簇通常呈球形，没有任何导管样延伸。使用一组单克隆和多克隆抗体进行的超微结构和免疫细胞化学研究表明，在原始乳腺组织中可识别的细胞类型和特征在子代群体中也可以描绘出来。由被单层内层上皮细胞和外层基底/肌上皮细胞包围的管腔组成的地形特征，可以从小细胞团开始在胶原凝胶系统中重新创建。
