转化生长因子α对使用三维胶原凝胶进行无血清原代培养的正常人乳腺上皮细胞生长的影响。

Effect of TGF-alpha on growth of normal human breast epithelial cells in serum-free primary culture using 3-dimensional collagen gels.

作者信息

Sakthivel R, Hamdan M, Yang J, Guzman R C, Nandi S

机构信息

Department of Molecular and Cell Biology, University of California, Berkeley 94720.

出版信息

Cell Biol Int. 1993 Apr;17(4):387-97. doi: 10.1006/cbir.1993.1077.

DOI:10.1006/cbir.1993.1077

PMID:8318950

Abstract

The mitogenic effect of TGF-alpha, acidic-FGF, basic-FGF and lithium on normal human breast epithelial cells was studied in a collagen gel culture system using a serum-free 1:1 mixture of Ham's F12 and DME medium containing insulin, cholera toxin and bovine serum albumin. TGF-alpha elicited a strong mitogenic response in a dose dependent manner. Addition of cortisol to TGF-alpha stimulated growth over and above that achieved with TGF-alpha alone. A consistent observation has been the effect of a combination of TGF-alpha and cortisol on growth stimulation of normal human breast epithelial cells resulting in 3-12 fold growth after 11-13 days in culture. Acidic-FGF, basic-FGF and lithium were not growth promoting.

摘要

在含有胰岛素、霍乱毒素和牛血清白蛋白的Ham's F12与DME培养基1:1无血清混合物的胶原凝胶培养系统中，研究了转化生长因子-α（TGF-α）、酸性成纤维细胞生长因子（acidic-FGF）、碱性成纤维细胞生长因子（basic-FGF）和锂对正常人乳腺上皮细胞的促有丝分裂作用。TGF-α以剂量依赖性方式引发强烈的促有丝分裂反应。向TGF-α中添加皮质醇比单独使用TGF-α能进一步刺激生长。一个一致的观察结果是，TGF-α和皮质醇联合作用对正常人乳腺上皮细胞的生长刺激作用，在培养11 - 13天后导致细胞生长3 - 12倍。酸性成纤维细胞生长因子、碱性成纤维细胞生长因子和锂不具有促进生长的作用。