硒与杜克雷嗜血杆菌的生长
Selenium and the growth of Haemophilus ducreyi.
作者信息
Vanden Berghe D A
机构信息
Laboratory of Microbiology, University of Antwerp, Belgium.
出版信息
J Clin Pathol. 1987 Oct;40(10):1174-7. doi: 10.1136/jcp.40.10.1174.
One of the growth media in current use for Haemophilus ducreyi comprises Mueller Hinton agar, chocolatised horse blood, serum and IsoVitalex (BBL). For a better understanding of growth factors, attempts were made to simplify this complex medium. The horse blood was replaced by haemin (200 micrograms/ml), the serum by albumin (0.2%), and IsoVitalex was substituted only by L-glutamine 0.01%. Most of the strains grew, but when selenium ions were added in a concentration of 3.25 x 10(-3) micrograms/ml, growth was stimulated and became more luxuriant than growth on conventional media.
目前用于杜克雷嗜血杆菌的一种生长培养基由穆勒-欣顿琼脂、巧克力化马血、血清和异维生素（BBL）组成。为了更好地理解生长因子，人们试图简化这种复杂的培养基。用血红素（200微克/毫升）替代马血，用白蛋白（0.2%）替代血清，仅用0.01%的L-谷氨酰胺替代异维生素。大多数菌株都能生长，但当添加浓度为3.25×10⁻³微克/毫升的硒离子时，生长受到刺激，且比在传统培养基上生长得更旺盛。
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