Yoshioka T, Bieberich C, Scangos G, Jay G
Laboratory of Molecular Virology, National Cancer Institute, Bethesda, MD 20892.
J Immunol. 1987 Dec 1;139(11):3861-7.
The function of a transgenic Dd class I molecule in the induction of immunologic tolerance to major histocompatibility complex antigens and in directing major histocompatibility complex restriction in C57BL/6 mice were investigated. All of the transgenic Dd mouse strains were found to be tolerant for the Dd antigen. Spleen cells from transgenic mice were immunocompetent but consistently failed to generate an anti-Dd cytotoxic T lymphocyte response in vitro, and skin grafts between transgenic Dd mice were not rejected. These data suggests that the Dd antigen was recognized as a self molecule. In addition, the transgenic Dd mice generated antigen-specific Dd-restricted cytotoxic T lymphocyte, indicating that the Dd antigen also functioned as a restriction element for antigen recognition. These observations demonstrate the usefulness of the transgenic mouse system for studying class I antigen expression and function.
研究了转基因Dd I类分子在诱导对主要组织相容性复合体抗原的免疫耐受以及指导C57BL/6小鼠主要组织相容性复合体限制方面的功能。发现所有转基因Dd小鼠品系对Dd抗原具有耐受性。转基因小鼠的脾细胞具有免疫活性，但在体外始终无法产生抗Dd细胞毒性T淋巴细胞反应，转基因Dd小鼠之间的皮肤移植也未被排斥。这些数据表明Dd抗原被识别为自身分子。此外，转基因Dd小鼠产生了抗原特异性Dd限制的细胞毒性T淋巴细胞，表明Dd抗原也作为抗原识别的限制元件发挥作用。这些观察结果证明了转基因小鼠系统在研究I类抗原表达和功能方面的实用性。
