Abanmy N, Alsabhan J, Gard P, Scutt G
College of Pharmacy, King Saud University, Riyadh 14212, Saudi Arabia.
School of Pharmacy and biomolecular sciences, University of Brighton, Brighton BN2 4AT, UK.
Saudi Pharm J. 2021 Dec;29(12):1392-1398. doi: 10.1016/j.jsps.2021.10.007. Epub 2021 Oct 25.
Brain derived neutrophic factor (BDNF) is a protein and a member of the neurotrophin family of growth factors, supports the survival of existing neurons and encourages the growth and differentiation of new neurons and synapses. The BDNF gene Val66Met polymorphism (rs6265/G196A) is responsible for BDNF synthesis that impact BDNF function that includes memory and cognition. This study investigated whether the BDNF gene Val66Met polymorphism (rs6265/G196A) is associated with cognitive function changes in both Alzheimer disease (AD) patients and elderly participants. In addition the impact of SSRI use on cognition improvement will be assessed. Healthy young, middle ages (25-59 years old) and elderly (more than 60 years old) participants (140) as well as 40 AD patients of whom are both of Saudi Arabian origin were recruited. The genotyping for the association study was performed by real-time PCR using Taqman chemistry in the ABI Prism 7900HT Sequence Detection System. Both Mini-Mental Status Examination (MMSE) and Clinical Dementia Rating (CDR) were used to assess cognitive function of healthy and AD participants, respectively. The findings showed that the BDNF Val66Met genotype distributions and allele frequencies have significant association with cognitive performance in both elderly control group and AD patients. The main findings showed that carriers of GG homozygotes (Val/Val) have superior cognitive performance among AD patients and elderly control subjects. In addition the use of SSRIs in 13 AD patients and 17 elderly participants positively improved cognitive function in elderly (p > 0.001) but not in AD patients (p = 0.1).
脑源性神经营养因子（BDNF）是一种蛋白质，属于生长因子的神经营养因子家族，它支持现有神经元的存活，并促进新神经元和突触的生长与分化。BDNF基因Val66Met多态性（rs6265/G196A）负责BDNF的合成，这会影响BDNF的功能，包括记忆和认知。本研究调查了BDNF基因Val66Met多态性（rs6265/G196A）是否与阿尔茨海默病（AD）患者和老年参与者的认知功能变化相关。此外，还将评估使用选择性5-羟色胺再摄取抑制剂（SSRI）对认知改善的影响。招募了140名健康的年轻人、中年（25 - 59岁）和老年人（60岁以上）参与者以及40名均为沙特阿拉伯血统的AD患者。关联研究的基因分型通过在ABI Prism 7900HT序列检测系统中使用Taqman化学方法的实时聚合酶链反应（PCR）进行。简易精神状态检查表（MMSE）和临床痴呆评定量表（CDR）分别用于评估健康参与者和AD患者的认知功能。研究结果表明，BDNF Val66Met基因型分布和等位基因频率与老年对照组和AD患者的认知表现均存在显著关联。主要研究结果表明，GG纯合子（Val/Val）携带者在AD患者和老年对照组中具有更优的认知表现。此外，13名AD患者和17名老年参与者使用SSRI后，老年参与者的认知功能得到了积极改善（p > 0.001），但AD患者未得到改善（p = 0.1）。
