Oyenihi Omolola R, Cerf Marlon E, Matsabisa Motlalepula G, Brooks Nicole L, Oguntibeju Oluwafemi O
Phytomedicine and Phytochemistry Group, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville 7535, South Africa.
Grants, Innovation and Product Development, South African Medical Research Council, Tygerberg, South Africa.
Saudi J Biol Sci. 2022 Jan;29(1):324-330. doi: 10.1016/j.sjbs.2021.08.095. Epub 2021 Sep 6.
Kolaviron, a biflavonoid isolated from the edible seeds of , lowers blood glucose in experimental models of diabetes; however, the underlying mechanisms are not yet fully elucidated. The objective of the current study was to assess the effects of kolaviron on islet dynamics in streptozotocin-induced diabetic rats. Using double immunolabeling of glucagon and insulin, we identified insulin-producing β- and glucagon-producing α-cells in the islets of diabetic and control rats and determined the fractional β-cell area, α-cell area and islet number. STZ challenged rats presented with islet hypoplasia and reduced β-cell area concomitant with an increase in α-cell area. Kolaviron restored some islet architecture in diabetic rats through the increased β-cell area. Overall, kolaviron-treated diabetic rats presented a significant (p < 0.05) increase in the number of large and very large islets compared to diabetic control but no difference in islet number and α-cell area. The β-cell replenishment potential of kolaviron and its overall positive effects on glycemic control suggest that it may be a viable target for diabetes treatment.
可乐维隆是从[植物名称]可食用种子中分离出的一种双黄酮，能降低糖尿病实验模型中的血糖；然而，其潜在机制尚未完全阐明。本研究的目的是评估可乐维隆对链脲佐菌素诱导的糖尿病大鼠胰岛动态的影响。通过对胰高血糖素和胰岛素进行双重免疫标记，我们在糖尿病大鼠和对照大鼠的胰岛中鉴定出产生胰岛素的β细胞和产生胰高血糖素的α细胞，并测定了β细胞面积分数、α细胞面积和胰岛数量。链脲佐菌素攻击的大鼠出现胰岛发育不全和β细胞面积减少，同时α细胞面积增加。可乐维隆通过增加β细胞面积恢复了糖尿病大鼠的一些胰岛结构。总体而言，与糖尿病对照组相比，经可乐维隆治疗的糖尿病大鼠的大胰岛和非常大的胰岛数量显著增加（p<0.05），但胰岛数量和α细胞面积没有差异。可乐维隆的β细胞补充潜力及其对血糖控制的总体积极作用表明，它可能是糖尿病治疗的一个可行靶点。
