Brüggemann M, Williams G T, Bindon C I, Clark M R, Walker M R, Jefferis R, Waldmann H, Neuberger M S
Department of Pathology, Addenbrooke's Hospital, Cambridge, United Kingdom.
J Exp Med. 1987 Nov 1;166(5):1351-61. doi: 10.1084/jem.166.5.1351.
Cell lines have been established that secrete a matched set of human chimeric IgM, IgG1, IgG2, IgG3, IgG4, IgE, and IgA2 antibodies that are directed against the hapten 4-hydroxy-3-nitrophenacetyl. These chimeric antibodies secreted from mouse plasmacytoma cells behave exactly like their authentic human counterparts in SDS-PAGE analysis, binding to protein A and in a wide range of serological assays. The antibodies have been compared in their ability to bind human C1q as well as in their efficacy in mediating lysis of human erythrocytes in the presence of human complement. A major conclusion to emerge is that whereas IgG3 bound C1q better than did IgG1, the chimeric IgG1 was much more effective than all the other IgG subclasses in complement-dependent hemolysis. The IgG1 antibody was also the most effective in mediating antibody-dependent cell-mediated cytotoxicity using both human effector and human target cells. These results suggest that IgG1 might be the favoured IgG subclass for therapeutic applications.
已经建立了一些细胞系，它们能分泌一组匹配的人源嵌合IgM、IgG1、IgG2、IgG3、IgG4、IgE和IgA2抗体，这些抗体针对半抗原4-羟基-3-硝基苯乙酰。从小鼠浆细胞瘤细胞分泌的这些嵌合抗体在SDS-PAGE分析、与蛋白A结合以及广泛的血清学检测中，其行为与真正的人源对应抗体完全一样。对这些抗体结合人C1q的能力以及在存在人补体的情况下介导人红细胞裂解的效力进行了比较。得出的一个主要结论是，虽然IgG3比IgG1更能结合C1q，但嵌合IgG1在补体依赖性溶血方面比所有其他IgG亚类都更有效。在使用人效应细胞和人靶细胞介导抗体依赖性细胞介导的细胞毒性方面，IgG1抗体也是最有效的。这些结果表明，IgG1可能是治疗应用中最受青睐的IgG亚类。
