• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

不同发育阶段桑叶的比较蛋白质组学鉴定出与光合作用相关的新功能蛋白质

Comparative Proteomics of Mulberry Leaves at Different Developmental Stages Identify Novel Proteins Function Related to Photosynthesis.

作者信息

Hou Zhiwei, Xu Dashun, Deng Na, Li Yan, Yang Luoling, Li Shuxuan, Zhou Hong, Huang Qintao, Wang Xiling

机构信息

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.

College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing, China.

出版信息

Front Plant Sci. 2021 Dec 24;12:797631. doi: 10.3389/fpls.2021.797631. eCollection 2021.

DOI:10.3389/fpls.2021.797631
PMID:35003187
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8739898/
Abstract

Mulberry leaves at different positions are different in photosynthetic rate, nutrient substance and feeding impact to silkworms. Here, we investigated the proteomic differences of the first (L1), sixth (L6), and twentieth (L20) mulberry leaves at different stem positions (from top to the base) using a label-free quantitative proteomics approach. L1 contained less developed photosynthetic apparatus but was more active in protein synthesis. L20 has more channel proteins and oxidoreductases relative to L6. Proteins that detected in all measured leaves were classified into three groups according to their expression patterns in L1, L6, and L20. The protein group that displayed the maximum amount in L6 has the highest possibility that function related to photosynthesis. Nine function unknown proteins belong to this group were further analyzed in the light responsive expression, evolutionary tree and sub-cellular localization analysis. Based on the results, five proteins were suggested to be involved in photosynthesis. Taken together, these results reveal the molecular details of different roles of mulberry leaves at different developmental stages and contribute to the identification of five proteins that might function related to photosynthesis.

摘要

不同位置的桑叶在光合速率、营养物质以及对家蚕的饲养影响方面存在差异。在此，我们采用无标记定量蛋白质组学方法，研究了不同茎部位置（从顶部到底部）的第一片（L1）、第六片（L6）和第二十片（L20）桑叶的蛋白质组差异。L1的光合器官发育程度较低，但蛋白质合成更为活跃。相对于L6，L20具有更多的通道蛋白和氧化还原酶。根据在L1、L6和L20中的表达模式，将在所有测定叶片中检测到的蛋白质分为三组。在L6中显示出最大量的蛋白质组与光合作用相关的功能可能性最高。对属于该组的九个功能未知蛋白质进行了光响应表达、进化树和亚细胞定位分析。基于这些结果，推测有五种蛋白质参与光合作用。综上所述，这些结果揭示了桑叶在不同发育阶段不同作用的分子细节，并有助于鉴定可能与光合作用相关的五种蛋白质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/304176861abc/fpls-12-797631-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/e43772720520/fpls-12-797631-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/0a275b398f70/fpls-12-797631-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/533db017b9b7/fpls-12-797631-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/573ca344bd9c/fpls-12-797631-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/e10ecf30005d/fpls-12-797631-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/f7e49b7bb04a/fpls-12-797631-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/b1ae8444fc90/fpls-12-797631-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/48a22ef8b4b6/fpls-12-797631-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/eccd7872f981/fpls-12-797631-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/789443f6dcdd/fpls-12-797631-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/304176861abc/fpls-12-797631-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/e43772720520/fpls-12-797631-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/0a275b398f70/fpls-12-797631-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/533db017b9b7/fpls-12-797631-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/573ca344bd9c/fpls-12-797631-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/e10ecf30005d/fpls-12-797631-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/f7e49b7bb04a/fpls-12-797631-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/b1ae8444fc90/fpls-12-797631-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/48a22ef8b4b6/fpls-12-797631-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/eccd7872f981/fpls-12-797631-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/789443f6dcdd/fpls-12-797631-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f67e/8739898/304176861abc/fpls-12-797631-g011.jpg

相似文献

1
Comparative Proteomics of Mulberry Leaves at Different Developmental Stages Identify Novel Proteins Function Related to Photosynthesis.不同发育阶段桑叶的比较蛋白质组学鉴定出与光合作用相关的新功能蛋白质
Front Plant Sci. 2021 Dec 24;12:797631. doi: 10.3389/fpls.2021.797631. eCollection 2021.
2
Physiological and comparative transcriptome analysis of the response and adaptation mechanism of the photosynthetic function of mulberry ( L.) leaves to flooding stress.对桑树（L.）叶片光合作用对水淹胁迫响应和适应机制的生理和比较转录组分析。
Plant Signal Behav. 2022 Dec 31;17(1):2094619. doi: 10.1080/15592324.2022.2094619.
3
iTRAQ-based analysis of developmental dynamics in the soybean leaf proteome reveals pathways associated with leaf photosynthetic rate.基于iTRAQ的大豆叶片蛋白质组发育动态分析揭示了与叶片光合速率相关的途径。
Mol Genet Genomics. 2016 Aug;291(4):1595-605. doi: 10.1007/s00438-016-1202-3. Epub 2016 Apr 5.
4
Comparative proteomic analysis provides new insights into mulberry dwarf responses in mulberry (Morus alba L.).比较蛋白质组学分析为桑树（Morus alba L.）矮化反应提供了新的见解。
Proteomics. 2009 Dec;9(23):5328-39. doi: 10.1002/pmic.200900012.
5
Physiological and proteomic responses of reactive oxygen species metabolism and antioxidant machinery in mulberry (Morus alba L.) seedling leaves to NaCl and NaHCO stress.NaCl 和 NaHCO 胁迫下桑树（Morus alba L.）幼苗叶片中活性氧代谢和抗氧化机制的生理和蛋白质组学响应。
Ecotoxicol Environ Saf. 2020 Apr 15;193:110259. doi: 10.1016/j.ecoenv.2020.110259. Epub 2020 Feb 22.
6
The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation.构树的冷响应机制：光合作用能力下降和淀粉积累增加。
BMC Genomics. 2015 Nov 5;16:898. doi: 10.1186/s12864-015-2047-6.
7
Toxic effects of heavy metals Pb and Cd on mulberry (Morus alba L.) seedling leaves: Photosynthetic function and reactive oxygen species (ROS) metabolism responses.重金属 Pb 和 Cd 对桑树（Morus alba L.）幼苗叶片的毒害作用：光合功能和活性氧（ROS）代谢响应。
Ecotoxicol Environ Saf. 2020 Jun 1;195:110469. doi: 10.1016/j.ecoenv.2020.110469. Epub 2020 Mar 13.
8
Comparative proteomics of leaves found at different stem positions of maize seedlings.玉米幼苗不同茎位叶片的比较蛋白质组学
J Plant Physiol. 2016 Jul 1;198:116-28. doi: 10.1016/j.jplph.2016.03.022. Epub 2016 Apr 30.
9
Comparative Transcriptome Analysis Identifies Key Defense Genes and Mechanisms in Mulberry () Leaves against Silkworms ().比较转录组分析鉴定了桑树（）叶片抵御家蚕（）的关键防御基因和机制。
Int J Mol Sci. 2022 Nov 4;23(21):13519. doi: 10.3390/ijms232113519.
10
Soybean (Glycine max L. Merr.) seedlings response to shading: leaf structure, photosynthesis and proteomic analysis.大豆（Glycine max L. Merr.）幼苗对遮荫的响应：叶片结构、光合作用和蛋白质组学分析。
BMC Plant Biol. 2019 Jan 21;19(1):34. doi: 10.1186/s12870-019-1633-1.

引用本文的文献

1
Transcriptome and metabolome analysis reveals different photosynthetic characteristics of mulberry trees with different ploidy levels.转录组和代谢组分析揭示了不同倍性水平桑树的不同光合特性。
Sci Rep. 2025 May 7;15(1):15892. doi: 10.1038/s41598-025-99598-1.

本文引用的文献

1
The Coordinated Upregulated Expression of Genes Involved in MEP, Chlorophyll, Carotenoid and Tocopherol Pathways, Mirrored the Corresponding Metabolite Contents in Rice Leaves during De-Etiolation.参与MEP、叶绿素、类胡萝卜素和生育酚途径的基因的协同上调表达，反映了水稻叶片在去黄化过程中相应的代谢物含量。
Plants (Basel). 2021 Jul 16;10(7):1456. doi: 10.3390/plants10071456.
2
Understanding photosynthesis in a spatial-temporal multiscale: The need for a systemic view.在时空多尺度上理解光合作用：需要系统的视角。
Theor Exp Plant Physiol. 2021;33(2):113-124. doi: 10.1007/s40626-021-00199-w. Epub 2021 Apr 4.
3
The physiological response of photosynthesis to nitrogen deficiency.
光合作用对氮缺乏的生理响应。
Plant Physiol Biochem. 2021 Jan;158:76-82. doi: 10.1016/j.plaphy.2020.11.019. Epub 2020 Nov 17.
4
Genotypic variation in the grain photosynthetic contribution to grain filling in rice.水稻灌浆期粒叶光合贡献的基因型变异。
J Plant Physiol. 2020 Oct;253:153269. doi: 10.1016/j.jplph.2020.153269. Epub 2020 Aug 28.
5
Physiological and proteomics responses of nitrogen assimilation and glutamine/glutamine family of amino acids metabolism in mulberry ( L.) leaves to NaCl and NaHCO stress.盐胁迫和碱胁迫下桑叶氮同化和谷氨酰胺/谷氨酰胺家族氨基酸代谢的生理和蛋白质组学响应。
Plant Signal Behav. 2020 Oct 2;15(10):1798108. doi: 10.1080/15592324.2020.1798108. Epub 2020 Jul 30.
6
The reduction in leaf area precedes that in photosynthesis under potassium deficiency: the importance of leaf anatomy.缺钾条件下叶面积的减小先于光合作用的减弱：叶片解剖结构的重要性
New Phytol. 2020 Sep;227(6):1749-1763. doi: 10.1111/nph.16644. Epub 2020 Jun 8.
7
Physiological and proteomic responses of reactive oxygen species metabolism and antioxidant machinery in mulberry (Morus alba L.) seedling leaves to NaCl and NaHCO stress.NaCl 和 NaHCO 胁迫下桑树（Morus alba L.）幼苗叶片中活性氧代谢和抗氧化机制的生理和蛋白质组学响应。
Ecotoxicol Environ Saf. 2020 Apr 15;193:110259. doi: 10.1016/j.ecoenv.2020.110259. Epub 2020 Feb 22.
8
Metabolic Profiling and Transcriptome Analysis of Mulberry Leaves Provide Insights into Flavonoid Biosynthesis.桑叶代谢组学和转录组学分析为类黄酮生物合成提供了新见解。
J Agric Food Chem. 2020 Feb 5;68(5):1494-1504. doi: 10.1021/acs.jafc.9b06931. Epub 2020 Jan 22.
9
A novel chlorophyll protein complex in the repair cycle of photosystem II.一种新型的叶绿素蛋白复合物在光系统 II 的修复循环中。
Proc Natl Acad Sci U S A. 2019 Oct 22;116(43):21907-21913. doi: 10.1073/pnas.1909644116. Epub 2019 Oct 8.
10
Improved estimates of global terrestrial photosynthesis using information on leaf chlorophyll content.利用叶片叶绿素含量信息改进全球陆地光合作用的估算。
Glob Chang Biol. 2019 Jul;25(7):2499-2514. doi: 10.1111/gcb.14624. Epub 2019 Apr 10.