Chen Yi-Bo, Wang Dan, Ge Xuan-Liang, Zhao Biligen-Gaowa, Wang Xu-Chu, Wang Bai-Chen
Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Xiangshan, Beijing, China.
Key Laboratory of Biology and Genetic Resources for Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.
J Plant Physiol. 2016 Jul 1;198:116-28. doi: 10.1016/j.jplph.2016.03.022. Epub 2016 Apr 30.
To better understand the roles of leaves at different stem positions during plant development, we measured the physiological properties of leaves 1-4 on maize seedling stems, and performed a proteomics study to investigate the differences in protein expression in the four leaves using two-dimensional difference gel electrophoresis and tandem mass spectrometry in conjunction with database searching. A total of 167 significantly differentially expressed protein spots were found and identified. Of these, 35% are involved in photosynthesis. By further analysis of the data, we speculated that in leaf 1 the seedling has started to transition from a heterotroph to an autotroph, development of leaf 2 is the time at which the seedling fully transitions from a heterotroph to an autotroph, and leaf maturity was reached only with fully expanded leaves 3 and 4, although there were still some protein expression differences in the two leaves. These results suggest that the different leaves make different contributions to maize seedling growth via modulation of the expression of the photosynthetic proteins. Together, these results provide insight into the roles of the different maize leaves as the plant develops from a heterotroph to an autotroph.
为了更好地了解植物发育过程中不同茎位叶片的作用，我们测定了玉米幼苗茎上第1 - 4片叶的生理特性，并进行了蛋白质组学研究，采用二维差异凝胶电泳和串联质谱联用数据库搜索的方法，研究这四片叶中蛋白质表达的差异。共发现并鉴定出167个显著差异表达的蛋白质斑点。其中，35%参与光合作用。通过对数据的进一步分析，我们推测在第1片叶时，幼苗已开始从异养向自养转变，第2片叶的发育是幼苗完全从异养转变为自养的时期，只有第3片和第4片叶完全展开时才达到叶片成熟，尽管这两片叶仍存在一些蛋白质表达差异。这些结果表明，不同叶片通过调节光合蛋白的表达对玉米幼苗生长做出不同贡献。总之，这些结果为了解不同玉米叶片在植物从异养向自养发育过程中的作用提供了见解。
