Kaur Vishaldeep, Sharma Mridu, Sen Tapasi
Institute of Nano Science and Technology, Mohali, India.
Front Chem. 2021 Dec 23;9:772267. doi: 10.3389/fchem.2021.772267. eCollection 2021.
The abundance of hotspots tuned via precise arrangement of coupled plasmonic nanostructures highly boost the surface-enhanced Raman scattering (SERS) signal enhancements, expanding their potential applicability to a diverse range of applications. Herein, nanoscale assembly of Ag coated Au nanostars in dimer and trimer configurations with tunable nanogap was achieved using programmable DNA origami technique. The resulting assemblies were then utilized for SERS-based ultra-sensitive detection of an important neurotransmitter, dopamine. The trimer assemblies were able to detect dopamine with picomolar sensitivity, and the assembled dimer structures achieved SERS sensitivity as low as 1 fM with a limit of detection of 0.225 fM. Overall, such coupled nanoarchitectures with superior plasmon tunability are promising to explore new avenues in biomedical diagnostic applications.
通过耦合等离子体纳米结构的精确排列调节的热点丰度极大地增强了表面增强拉曼散射（SERS）信号，拓展了其在各种应用中的潜在适用性。在此，利用可编程DNA折纸技术实现了具有可调纳米间隙的二聚体和三聚体构型的银包覆金纳米星的纳米级组装。然后将所得组件用于基于SERS的重要神经递质多巴胺的超灵敏检测。三聚体组件能够以皮摩尔灵敏度检测多巴胺，组装的二聚体结构实现了低至1 fM的SERS灵敏度，检测限为0.225 fM。总体而言，这种具有卓越等离子体可调性的耦合纳米结构有望在生物医学诊断应用中探索新途径。
