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京尼平交联脱细胞髓核类水凝胶细胞递送系统诱导脂肪干细胞分化并延缓椎间盘退变。

Genipin Cross-Linked Decellularized Nucleus Pulposus Hydrogel-Like Cell Delivery System Induces Differentiation of ADSCs and Retards Intervertebral Disc Degeneration.

作者信息

Yu Lei, Liu Yi, Wu Jianxin, Wang Shuang, Yu Jiangming, Wang Weiheng, Ye Xiaojian

机构信息

Department of Orthopedics, Second Affiliated Hospital of Naval Medical University, Shanghai, China.

Department of Orthopedics, First Affiliated Hospital of Naval Medical University, Shanghai, China.

出版信息

Front Bioeng Biotechnol. 2021 Dec 23;9:807883. doi: 10.3389/fbioe.2021.807883. eCollection 2021.

DOI:10.3389/fbioe.2021.807883
PMID:35004657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8733700/
Abstract

Intervertebral disc degeneration (IDD) is the pathological basis of disc degenerative diseases (DDD). Reduction in the number of cells and degeneration of the extracellular matrix (ECM) in the nucleus pulposus (NP) are characteristics of IDD. Bio-hydrogel combined with stem cell transplantation is a promising treatment. Injectable ECM hydrogels have good biological activity and gelatinization. However, its biomechanics and stability are insufficient to provide adequate mechanical support for intervertebral discs and to maintain the long-term differential stimulus for seeded stem cells. In our study, we developed genipin cross-linked decellularized nucleus pulposus hydrogel (GDH) as delivery system. We evaluated the mechanical properties, stability, biocompatibility, and differentiation induction of GDH cross-linked with different concentrations of genipin . The GDH-loaded adipose-derived mesenchymal stem cells (ADSCs) (GDHA) were injected into the rat degenerated coccygeal intervertebral disc. The effect of intervertebral disc regeneration was evaluated. The results showed that GDH with 0.02% of genipin had similar elastic modulus to human nucleus pulposus, good biocompatibility, and inducibility of expressing NP-related genes. studies showed that GDHA improved the survival of ADSCs and improved the intervertebral height, MRI index, and histological grading score. In conclusion, GDH, as an outstanding bio-hydrogel cell delivery system, has the therapeutic potential for retarding IDD.

摘要

椎间盘退变（IDD）是椎间盘退变性疾病（DDD）的病理基础。髓核（NP）中细胞数量减少和细胞外基质（ECM）退变是IDD的特征。生物水凝胶联合干细胞移植是一种有前景的治疗方法。可注射的ECM水凝胶具有良好的生物活性和凝胶化特性。然而，其生物力学性能和稳定性不足以对椎间盘提供足够的机械支撑，也无法为接种的干细胞维持长期的差异刺激。在我们的研究中，我们开发了京尼平交联的脱细胞髓核水凝胶（GDH）作为递送系统。我们评估了与不同浓度京尼平交联的GDH的力学性能、稳定性、生物相容性和分化诱导能力。将负载有脂肪来源间充质干细胞（ADSCs）的GDH（GDHA）注入大鼠退变的尾椎椎间盘。评估椎间盘再生的效果。结果表明，含0.02%京尼平的GDH具有与人髓核相似的弹性模量、良好的生物相容性以及表达NP相关基因的诱导能力。研究表明，GDHA提高了ADSCs的存活率，并改善了椎间盘高度、MRI指数和组织学分级评分。总之，GDH作为一种出色的生物水凝胶细胞递送系统，具有延缓IDD的治疗潜力。

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本文引用的文献

1
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Differentiation. 2021 Nov-Dec;122:1-6. doi: 10.1016/j.diff.2021.11.001. Epub 2021 Nov 3.
2
Degenerative physiochemical events in the pathological intervertebral disc.病理性椎间盘的退行性理化事件。
Histol Histopathol. 2022 Jan;37(1):11-20. doi: 10.14670/HH-18-395. Epub 2021 Nov 10.
3
Stem Cell Therapy and Exercise for Treatment of Intervertebral Disc Degeneration.干细胞疗法与运动治疗椎间盘退变
机器学习和单细胞分析确定线粒体自噬相关基因TOMM22作为椎间盘退变的潜在诊断生物标志物。
Heliyon. 2024 Sep 3;10(17):e37378. doi: 10.1016/j.heliyon.2024.e37378. eCollection 2024 Sep 15.
4
AR regulate inflammation through PKA/NF-κB signaling pathways in intervertebral disc degeneration.AR 通过 PKA/NF-κB 信号通路调节椎间盘退变中的炎症反应。
Eur J Med Res. 2024 Aug 27;29(1):433. doi: 10.1186/s40001-024-02028-7.
5
Progress in the Application of Hydrogels in Intervertebral Disc Repair: A Comprehensive Review.水凝胶在椎间盘修复中的应用进展：综述
Curr Pain Headache Rep. 2024 Dec;28(12):1333-1348. doi: 10.1007/s11916-024-01296-6. Epub 2024 Jul 10.
6
Modulation of Decellularized Lacrimal Gland Hydrogel Biodegradation by Genipin Crosslinking.基因素交联调节去细胞化的泪腺水凝胶的生物降解。
Invest Ophthalmol Vis Sci. 2024 May 1;65(5):24. doi: 10.1167/iovs.65.5.24.
7
Adipose-derived stem cell-based optimization strategies for musculoskeletal regeneration: recent advances and perspectives.基于脂肪干细胞的肌肉骨骼再生优化策略：最新进展与展望。
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8
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Stem Cells Int. 2021 Oct 13;2021:7982333. doi: 10.1155/2021/7982333. eCollection 2021.
4
Global estimates of the need for rehabilitation based on the Global Burden of Disease study 2019: a systematic analysis for the Global Burden of Disease Study 2019.基于 2019 年全球疾病负担研究的全球康复需求估计：2019 年全球疾病负担研究的系统分析。
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5
Biomaterials Regulate Mechanosensors YAP/TAZ in Stem Cell Growth and Differentiation.生物材料调节干细胞生长和分化中的机械感受器 YAP/TAZ。
Tissue Eng Regen Med. 2021 Apr;18(2):199-215. doi: 10.1007/s13770-020-00301-4. Epub 2020 Nov 24.
6
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J Biomech. 2020 Dec 2;113:110100. doi: 10.1016/j.jbiomech.2020.110100. Epub 2020 Oct 28.
7
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Front Bioeng Biotechnol. 2020 Jun 30;8:745. doi: 10.3389/fbioe.2020.00745. eCollection 2020.
8
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J Biomater Appl. 2020 Aug;35(2):182-192. doi: 10.1177/0885328220921328. Epub 2020 Apr 26.
9
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10
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