Scott H, Sollid L M, Fausa O, Brandtzaeg P, Thorsby E
Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Rikshospitalet, Oslo, Norway.
Scand J Immunol. 1987 Nov;26(5):563-71. doi: 10.1111/j.1365-3083.1987.tb02290.x.
The MHC class II subregion products (HLA-DR), HLA-DP, and HLA-DQ) were located by immunofluorescence in serial sections of ethanol-fixed, paraffin-embedded jejunal mucosa from control subjects and patients with coeliac disease (CD). DR staining was seen in a granular luminal distribution and basolaterally on surface epithelial cells in both untreated and treated CD patients and in controls. In untreated CD the crypt epithelium was positive for DR almost to the bottom of the glands. This contrasted with virtually absent glandular DR staining in controls and weak staining including only the upper part of the crypts in 5 out of 11 treated patients. HLA-DP was present apically in the surface epithelium in all untreated patients, in 5 out of 11 treated patients, and in 4 out of 11 controls. HLA-DQ appeared only in three untreated patients and was restricted to patches of surface epithelium. The number of intraepithelial T lymphocytes per millimetre of surface epithelium was significantly higher in untreated than in treated CD patients or controls; it was also significantly higher in specimens with epithelial DP expression than in those without. This suggested that intraepithelial lymphocytes modulate epithelial class II expression.
通过免疫荧光法，在对照受试者以及患有乳糜泻（CD）的患者经乙醇固定、石蜡包埋的空肠黏膜连续切片中定位了MHC II类亚区产物（HLA - DR、HLA - DP和HLA - DQ）。在未经治疗和经治疗的CD患者以及对照中，均可见DR染色呈颗粒状分布于管腔，并在表面上皮细胞的基底外侧。在未经治疗的CD中，隐窝上皮直至腺体底部DR均呈阳性。这与对照中几乎不存在腺体内DR染色形成对比，并且在11例经治疗的患者中有5例仅在隐窝上部有微弱染色。在所有未经治疗的患者、11例经治疗的患者中的5例以及11例对照中的4例中，HLA - DP存在于表面上皮的顶端。HLA - DQ仅在3例未经治疗的患者中出现，且局限于表面上皮的斑块处。每毫米表面上皮内的上皮内T淋巴细胞数量，未经治疗的CD患者显著高于经治疗的CD患者或对照；在有上皮DP表达的标本中也显著高于无上皮DP表达的标本。这表明上皮内淋巴细胞调节上皮II类分子的表达。
