Kehrl J H, Alvarez-Mon M, Delsing G A, Fauci A S
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Science. 1987 Nov 20;238(4830):1144-6. doi: 10.1126/science.3500512.
Two different assays for B cell growth factors (BCGF) and an antibody against lymphotoxin were used to show that the presence of lymphotoxin in conditioned media derived from normal activated T cells and in a partially purified BCGF accounts for a substantial portion of their B cell growth-promoting activity. A competitive binding assay confirmed the presence of significant amounts of lymphotoxin in the partially purified BCGF. Recombinant lymphotoxin enhanced the proliferation of activated B cells and augmented B cell proliferation and immunoglobulin secretion induced by interleukin-2.
采用两种不同的B细胞生长因子（BCGF）检测方法和一种抗淋巴毒素抗体，以证明来自正常活化T细胞的条件培养基和部分纯化的BCGF中存在的淋巴毒素，占其促进B细胞生长活性的很大一部分。竞争性结合试验证实部分纯化的BCGF中存在大量淋巴毒素。重组淋巴毒素增强了活化B细胞的增殖，并增强了白细胞介素-2诱导的B细胞增殖和免疫球蛋白分泌。
