Bentley Rachel E T, Hindmarch Charles C T, Dunham-Snary Kimberly J, Snetsinger Brooke, Mewburn Jeffrey D, Thébaud Arthur, Lima Patricia D A, Thébaud Bernard, Archer Stephen L
Department of Medicine, Queen's University, Kingston, Ontario, Canada.
QCPU, Queen's Cardiopulmonary Unit, Translational Institute of Medicine (TIME), Department of Medicine, Queen's University, Canada.
Data Brief. 2021 Dec 20;40:107736. doi: 10.1016/j.dib.2021.107736. eCollection 2022 Feb.
The Ductus Arteriosus (DA) is a fetal vessel that connects the aorta to the pulmonary artery ensuring that placental oxygenated blood is diverted from the lungs to the systemic circulation. Following exposure to oxygen (O), in the first few days of life, the DA responds with a functional closure that is followed by anatomical closure. Here, we study human DA smooth muscle cells (DASMC) taken from 10 term infants during congenital heart surgery. Purification of these cells using flow cytometry ensured a pure population of DASMCs, which we confirmed as responsive to O. An oxygen-induced increase in intracellular calcium of 18.1%±4.4% and SMC constriction (-27%±1.5% shortening) occurred in all cell lines within five minutes. These cells were maintained in either hypoxia (2.5% O), mimicking conditions or in normoxia (19% O) mimicking neonate conditions. We then used 3' RNAsequencing to identify the transcriptome of DASMCs in each condition [1]. In this paper, we present the full differentially regulated gene list from this experiment.
动脉导管（DA）是一种胎儿血管，它连接主动脉和肺动脉，确保胎盘氧合血从肺部转移至体循环。在出生后的头几天暴露于氧气（O₂）后，动脉导管会发生功能性关闭，随后是解剖学上的关闭。在此，我们研究了在先天性心脏手术期间从10名足月儿获取的人动脉导管平滑肌细胞（DASMC）。使用流式细胞术纯化这些细胞可确保获得纯的DASMC群体，我们证实这些细胞对O₂有反应。在所有细胞系中，五分钟内细胞内钙因氧气诱导增加了18.1%±4.4%，平滑肌收缩（缩短-27%±1.5%）。这些细胞被维持在模拟条件的低氧（2.5% O₂）或模拟新生儿条件的常氧（19% O₂）环境中。然后，我们使用3' RNA测序来鉴定每种条件下DASMC的转录组[1]。在本文中，我们展示了该实验中完整的差异调节基因列表。
