Department of Materials Science & Engineering, University of Toronto, Toronto M5S 3E4, Canada.
Food Science & Technology Department, Alexandria University, Alexandria 21526, Egypt.
ACS Appl Bio Mater. 2021 Dec 20;4(12):8248-8258. doi: 10.1021/acsabm.1c00794. Epub 2021 Nov 17.
Implanted medical devices such as central venous catheters are highly susceptible to microbial colonization and biofilm formation and are a major risk factor for nosocomial infections. The opportunistic pathogen uses exopolysaccharides, such as Psl, for both initial surface attachment and biofilm formation. We have previously shown that chemically immobilizing the Psl-specific glycoside hydrolase, PslG, to a material surface can inhibit biofilm formation. Herein, we show that PslG can be uniformly immobilized on the lumen surface of medical-grade, commercial polyethylene, polyurethane, and polydimethylsiloxane (silicone) catheter tubing. We confirmed that the surface-bound PslG was uniformly distributed along the catheter length and remained active even after storage for 30 days at 4 °C. colonization and biofilm formation under dynamic flow culture conditions showed a 3-log reduction in the number of bacteria during the first 11 days, and a 2-log reduction by day 14 for PslG-modified PE-100 catheters, compared to untreated catheter controls. In an rat infection model, PslG-modified PE-100 catheters showed a ∼1.5-log reduction in the colonization of the clinical ATCC 27853 strain after 24 h. These results demonstrate the robust ability of surface-bound glycoside hydrolase enzymes to inhibit biofilm formation and their potential to reduce rates of device-associated infections.
植入式医疗器械,如中心静脉导管,极易受到微生物的定殖和生物膜的形成,是医院感染的主要危险因素。机会性病原体利用多糖,如 Psl,进行初始表面附着和生物膜形成。我们之前已经表明,通过化学固定 Psl 特异性糖苷水解酶 PslG 到材料表面可以抑制生物膜的形成。在此,我们表明 PslG 可以均匀地固定在医用级商业聚乙烯、聚氨基甲酸酯和聚二甲基硅氧烷(硅酮)导管管腔表面。我们确认表面结合的 PslG 均匀分布在导管长度上,即使在 4°C 下储存 30 天后仍保持活性。在动态流动培养条件下的定殖和生物膜形成显示,在第 11 天之前,PslG 修饰的 PE-100 导管上的细菌数量减少了 3 个对数级,在第 14 天减少了 2 个对数级,而未处理的导管对照则减少了 3 个对数级。在大鼠感染模型中,与未经处理的导管对照相比,在 24 小时后,PslG 修饰的 PE-100 导管上临床 ATCC 27853 菌株的定植减少了约 1.5 个对数级。这些结果表明,表面结合的糖苷水解酶具有强大的抑制生物膜形成的能力,并且有可能降低器械相关感染的发生率。