Yin Fan, Qian Han, Duan Caiwen, Ning Botao
Department of Pediatric Intensive Care Unit, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, 1678 Dongfang Road, Shanghai, China.
Department of Translational Institution, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, 1678 Dongfang Road, Shanghai, China.
Mol Biomed. 2020 Oct 20;1(1):10. doi: 10.1186/s43556-020-00010-3.
Multiple organ dysfunction is an important cause of death in patients with sepsis. Currently, few studies have focused on the impact of sepsis on bone marrow (BM), especially on the cell components of BM niche. In this study, we performed mouse sepsis models by intraperitoneal injection of LPS and cecal ligation and puncture (CLP). The changes of niche major components in the mouse BM among vascular structures, mesenchymal stem cells and Treg cells were observed and analyzed. The results showed that pathological changes in BM was earlier and more prominent than in other organs, and various cell components of the BM niche changed significantly, of which vascular endothelial cells increased transiently with vascular remodeling and the regulatory T cells decreased over a long period of time. These results indicated that the components of the BM niche underwent series of adaptive changes in sepsis.
多器官功能障碍是脓毒症患者死亡的重要原因。目前，很少有研究关注脓毒症对骨髓（BM）的影响，尤其是对骨髓生态位细胞成分的影响。在本研究中，我们通过腹腔注射脂多糖（LPS）和盲肠结扎穿刺（CLP）建立小鼠脓毒症模型。观察并分析了小鼠骨髓中生态位主要成分在血管结构、间充质干细胞和调节性T细胞方面的变化。结果显示，骨髓的病理变化比其他器官更早且更显著，骨髓生态位的各种细胞成分发生了显著变化，其中血管内皮细胞随着血管重塑而短暂增加，调节性T细胞长期减少。这些结果表明，脓毒症时骨髓生态位的成分发生了一系列适应性变化。
