Department of Chemistry and the Texas Center for Superconductivity, University of Houston, 4800 Calhoun Road, Houston, Texas 77204-5003, United States.
Department of Biology and Biochemistry, University of Houston, 4800 Calhoun Road, Houston, Texas 77204-5001, United States.
ACS Appl Bio Mater. 2021 Feb 15;4(2):1563-1572. doi: 10.1021/acsabm.0c01409. Epub 2021 Feb 1.
Biofouling negatively impacts modern society on a daily basis, especially with regard to the important industries of medicine, oil, and shipping. This manuscript describes the preparation and study of model antifouling coatings generated from the adsorption of unsymmetrical partially fluorinated spiroalkanedithiols on gold. The antifouling properties of the self-assembled monolayers (SAMs) derived from the spiroalkanedithiols were compared to SAMs derived from analogous monodentate partially fluorinated and nonfluorinated alkanethiols. The antifouling properties were evaluated using surface plasmon resonance spectroscopy (SPR), electrochemical quartz crystal microbalance (QCM) measurements, and ellipsometric thickness measurements. The resistance to nonspecific protein adsorption of the SAMs was evaluated with proteins having a wide range of properties and applications including protamine, lysozyme, bovine serum albumin, and fibrinogen. The results from the SPR and the QCM measurements demonstrated that in most cases, the SAM coatings derived from the partially fluorinated spiroalkanedithiols having mixed hydrocarbon and fluorocarbon tail groups exhibited better antifouling performance when compared to the SAMs derived from their single-component monodentate counterparts. The studies also revealed that while the SPR and the QCM measurements in most cases were able to distinguish the adsorption trends for the SAMs and proteins examined, the ellipsometric thickness measurements were markedly less discriminating. On the whole, these studies validate the use of unsymmetrical partially fluorinated spiroalkanedithiols for generating effective antifouling coatings on metal substrates.
生物污垢每天都在对现代社会造成负面影响，尤其是在医学、石油和航运等重要行业。本文描述了模型防污涂层的制备和研究，该模型防污涂层是通过不对称部分氟化螺烷二硫醇在金上的吸附生成的。将螺烷二硫醇衍生的自组装单层（SAM）的防污性能与类似的单齿部分氟化和非氟化烷硫醇衍生的 SAM 进行了比较。使用表面等离子体共振光谱（SPR）、电化学石英晶体微天平（QCM）测量和椭圆光度厚度测量来评估防污性能。通过具有广泛性质和应用的蛋白质（包括鱼精蛋白、溶菌酶、牛血清白蛋白和纤维蛋白原）评估 SAM 对非特异性蛋白质吸附的抵抗力。SPR 和 QCM 测量的结果表明，在大多数情况下，与单组分单齿对应物相比，具有混合碳氢化合物和氟碳尾基团的部分氟化螺烷二硫醇衍生的 SAM 涂层在大多数情况下表现出更好的防污性能。研究还表明，尽管在大多数情况下，SPR 和 QCM 测量能够区分 SAM 和蛋白质的吸附趋势，但椭圆光度厚度测量的区分能力明显较差。总的来说，这些研究验证了使用不对称部分氟化螺烷二硫醇在金属基底上生成有效防污涂层的方法。
