Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
Ludwig Institute for Cancer Research, 1066 Lausanne, & Department of Oncology University of Lausanne & University Hospital of Lausanne (CHUV), 1066 Lausanne, Switzerland.
ACS Sens. 2022 Jan 28;7(1):159-165. doi: 10.1021/acssensors.1c01935. Epub 2022 Jan 10.
We developed an integrated microfluidic cellular processing unit (mCPU) capable of autonomously isolating single cells and performing measurements and on-the-fly analysis of cell-surface dissociation rates, followed by recovery of selected cells. We performed proof-of-concept, high-throughput single-cell experiments characterizing pMHC-TCR interactions on live CD8+ T cells. The mCPU platform analyzed TCR-pMHC dissociation rates with a throughput of 50 cells per hour and hundreds of cells per run, and we demonstrate that cells can be selected, enriched, and easily recovered from the device.
我们开发了一种集成的微流控细胞处理单元（mCPU），能够自主分离单细胞，并对细胞表面解离率进行测量和实时分析，随后回收选定的细胞。我们进行了概念验证的高通量单细胞实验，对活 CD8+T 细胞上的 pMHC-TCR 相互作用进行了表征。mCPU 平台以每小时 50 个细胞和每次运行数百个细胞的通量分析 TCR-pMHC 解离率，并且我们证明可以从设备中选择、富集和轻松回收细胞。
