Intraperitoneal Injection of Cyanine-Based Nanomicelles for Enhanced Near-Infrared Fluorescence Imaging and Surgical Navigation in Abdominal Tumors.

Fluorescent surgical navigation can effectively aid tumor resection. As one of the most popular near-infrared (NIR) fluorophores, cyanine dye has the outstanding optical ability and the potential to act as a fluorescence probe for tumors. Herein, we report a polyethylene glycol-modified amphiphilic cyanine dye (Cy7-NPC) with an NIR luminescence performance, which can self-assemble to form uniform nanomicelles (Cy7-NPC-S) and which can be applied for the optical imaging of abdominal tumors and for fluorescence imaging-guided precision tumor resection. When applied to biological imaging, Cy7-NPC-S showed high biological safety, strong tissue penetration depth for optical imaging, and high optical imaging resolution. Intraperitoneal administration of Cy7-NPC-S produced remarkable imaging efficacy in abdominal tumors. Compared with intravenous injection, abdominal tumors took up intraperitoneal Cy7-NPC-S faster and in greater quantities, thus enabling Cy7-NPC-S to facilitate accurate recognition and extirpation of abdominal tumors in fluorescence-guided surgery. We believe that metabolizable Cy7-NPC-S with NIR luminescence has promising applications and value in the fields of imaging and fluorescent surgical navigation.