Department of Applied Chemistry, National Yang Ming Chiao Tung University, Hsinchu, Taiwan 300.
ACS Appl Bio Mater. 2021 Nov 15;4(11):7893-7902. doi: 10.1021/acsabm.1c00817. Epub 2021 Oct 27.
Infectious diseases triggered by bacteria cause a severe risk to human health. To counter this issue, surfaces coated with antibacterial materials have been widely used in daily life to kill these bacteria. The substrates enabled with a hybrid kill and release strategy can be employed not only to kill the bacteria but also to wash them using external stimuli (temperature, pH, etc.). Utilizing this concept, we develop thermoresponsive antibacterial-cellulose papers to exhibit hybrid kill and release properties. Thermoresponsive copolymers [p(NIPAAm--AEMA)] are grafted on cellulose papers using a surface-initiated atom transfer radical polymerization approach for bacterial debris release. Later for antibacterial properties, silver nanoparticles (AgNPs) are immobilized on thermoresponsive copolymer-grafted cellulose papers using electrostatic interactions. We confirm the thermoresponsive copolymer grafting and AgNP coating by attenuated total reflection Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy. Thermoresponsiveness and reusability of the modified cellulose papers are confirmed through water contact angle measurements. The interaction potency between AgNPs and modified cellulose is validated by inductively coupled plasma atomic emission spectroscopy analysis. Gram-negative bacteria ( DH5-α) is used to demonstrate antibacterial hybrid kill and release performance. Agar-diffusion testing demonstrates the antibacterial nature of the modified cellulose papers. The fluorescence micrograph reveals that modified cellulose papers can effectively release almost all the dead bacterial debris from their surfaces after thermal stimulus wash. The modified cellulose paper surfaces are expected to have wide applications in the field of exploring more antibacterial and smart surfaces.
细菌引发的传染病对人类健康构成严重威胁。为了解决这个问题，日常生活中广泛使用了涂有抗菌材料的表面来杀死这些细菌。具有混合杀伤和释放策略的基底不仅可以用来杀死细菌，还可以使用外部刺激（温度、pH 值等）将其冲洗掉。利用这一概念，我们开发了热响应抗菌纤维素纸，以表现出混合杀伤和释放特性。使用表面引发原子转移自由基聚合方法将温敏共聚物 [p（NIPAAm-AEMA）] 接枝在纤维素纸上，以释放细菌碎片。后来为了抗菌性能，使用静电相互作用将银纳米粒子（AgNPs）固定在温敏共聚体接枝的纤维素纸上。我们通过衰减全反射傅里叶变换红外光谱、X 射线光电子能谱和扫描电子显微镜证实了温敏共聚体接枝和 AgNP 涂层。通过水接触角测量证实了改性纤维素纸的热响应性和可重复使用性。通过电感耦合等离子体原子发射光谱分析验证了 AgNPs 和改性纤维素之间的相互作用强度。革兰氏阴性菌（DH5-α）被用来证明改性纤维素纸的抗菌混合杀伤和释放性能。琼脂扩散试验证明了改性纤维素纸的抗菌性质。荧光显微镜照片显示，经过热刺激洗涤后，改性纤维素纸可以有效地从其表面释放几乎所有死亡的细菌碎片。改性纤维素纸表面有望在探索更多抗菌和智能表面的领域得到广泛应用。
