Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, P. R. China.
Shanxi Key Laboratory of Material Strength & Structural Impact, Institute of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, P. R. China.
ACS Appl Bio Mater. 2021 Aug 16;4(8):6167-6174. doi: 10.1021/acsabm.1c00493. Epub 2021 Jul 30.
The repair of bone defects is one of the great challenges facing modern orthopedics clinics. Bone tissue engineering scaffold with a nanofibrous structure similar to the original microstructure of a bone is beneficial for bone tissue regeneration. Here, a core-shell nanofibrous membrane (MS), MS containing glucosamine (MS-GLU), MS with a shish-kebab (SK) structure (SKMS), and MS-GLU with a SK structure (SKMS-GLU) were prepared by micro-sol electrospinning technology and a self-induced crystallization method. The diameter of MS nanofibers was 50-900 nm. Contact angle experiments showed that the hydrophilicity of SKMS was moderate, and its contact angle was as low as 72°. SK and GLU have a synergistic effect on cell growth. GLU in the core of MS was demonstrated to obviously promote MC3T3-E1 cell proliferation. At the same time, the SK structure grown on MS-GLU nanofibers mimicked natural collagen fibers, which facilitated MC3T3-E1 cell adhesion and differentiation. This study showed that a biomimetic SKMS-GLU nanofibrous membrane was a promising tissue engineering scaffold for bone defect repair.
骨缺损的修复是现代骨科临床面临的重大挑战之一。具有类似骨原始微观结构的纳米纤维结构的骨组织工程支架有利于骨组织再生。在这里，通过微溶胶静电纺丝技术和自诱导结晶法制备了核壳纳米纤维膜（MS）、含有葡糖胺的 MS（MS-GLU）、具有 shish-kebab（SK）结构的 MS（SKMS）和 MS-GLU 具有 SK 结构（SKMS-GLU）。MS 纳米纤维的直径为 50-900nm。接触角实验表明，SKMS 的亲水性适中，其接触角低至 72°。SK 和 GLU 对细胞生长具有协同作用。MS 中心的 GLU 明显促进了 MC3T3-E1 细胞的增殖。同时，生长在 MS-GLU 纳米纤维上的 SK 结构模拟了天然胶原纤维，有利于 MC3T3-E1 细胞的黏附和分化。本研究表明，仿生 SKMS-GLU 纳米纤维膜是一种很有前途的骨缺损修复组织工程支架。
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