Yin Haiyue, Wang Juan, Gu Ziqi, Feng Wenhao, Gao Manchen, Wu Yu, Zheng Hao, He Xiaomin, Mo Xiumei
1 Key Laboratory of Science & Technology of Eco-Textile, Ministry of Education College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, China.
2 Department of Pediatric Cardiothoracic Surgery, Shanghai Children's Medical Center affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
J Biomater Appl. 2017 Sep;32(3):331-341. doi: 10.1177/0885328217717077. Epub 2017 Jun 28.
Tracheal stenosis is one of major challenging issues in clinical medicine because of the poor intrinsic ability of tracheal cartilage for repair. Tissue engineering provides an alternative method for the treatment of tracheal defects by generating replacement tracheal structures. In this study, we fabricated coaxial electrospun fibers using poly(L-lactic acid-co-caprolactone) and collagen solution as shell fluid and kartogenin solution as core fluid. Scanning electron microscope and transmission electron microscope images demonstrated that nanofibers had uniform and smooth structure. The kartogenin released from the scaffolds in a sustained and stable manner for about 2 months. The bioactivity of released kartogenin was evaluated by its effect on maintain the synthesis of type II collagen and glycosaminoglycans by chondrocytes. The proliferation and morphology analyses of mesenchymal stems cells derived from bone marrow of rabbits indicated the good biocompatibility of the fabricated nanofibrous scaffold. Meanwhile, the chondrogenic differentiation of bone marrow mesenchymal stem cells cultured on core-shell nanofibrous scaffold was evaluated by real-time polymerase chain reaction. The results suggested that the core-shell nanofibrous scaffold with kartogenin could promote the chondrogenic differentiation ability of bone marrow mesenchymal stem cells. Overall, the core-shell nanofibrous scaffold could be an effective delivery system for kartogenin and served as a promising tissue engineered scaffold for tracheal cartilage regeneration.
由于气管软骨自身修复能力较差，气管狭窄是临床医学中主要的挑战性问题之一。组织工程通过生成替代气管结构，为气管缺损的治疗提供了一种替代方法。在本研究中，我们使用聚（L-乳酸-共-己内酯）和胶原溶液作为壳层流体，卡托金溶液作为芯层流体，制备了同轴电纺纤维。扫描电子显微镜和透射电子显微镜图像显示，纳米纤维结构均匀且光滑。卡托金从支架中持续稳定释放约2个月。通过其对维持软骨细胞合成II型胶原和糖胺聚糖的作用，评估释放的卡托金的生物活性。对兔骨髓来源的间充质干细胞进行增殖和形态分析，表明所制备的纳米纤维支架具有良好的生物相容性。同时，通过实时聚合酶链反应评估在核壳纳米纤维支架上培养的骨髓间充质干细胞的软骨分化情况。结果表明，含有卡托金的核壳纳米纤维支架可促进骨髓间充质干细胞的软骨分化能力。总体而言，核壳纳米纤维支架可能是一种有效的卡托金递送系统，有望成为用于气管软骨再生的组织工程支架。
