Université de Limoges, Laboratoire PEIRENE, EA 7500, Faculté des Sciences et Techniques, 123 Avenue Albert Thomas, 87060 Limoges Cedex, France.
Université de Limoges, IRCER, UMR 7315, F-87068 Limoges, France.
ACS Appl Bio Mater. 2021 Sep 20;4(9):7204-7212. doi: 10.1021/acsabm.1c00760. Epub 2021 Sep 2.
Photodynamic antimicrobial chemotherapy or PACT has been shown to be a promising antibacterial treatment that could overcome the challenge of multidrug-resistant bacteria. However, the use of most existing photosensitizers has been severely hampered by their significant self-quenching effect, poor water solubility, lack of selectivity against bacterial cells, and possible damage to the surrounding tissues. The use of hydrogels may overcome some of these limitations. We herein report a simple strategy to synthesize a cross-linked hydrogel from beech xylan. The hydrogel showed a high swelling ratio, up to 62, an interconnected porous structure, and good mechanical integrity, and 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin tetraiodide (TMPyP) was chosen as a model of hydrophilic photosensitizer (PS) and was encapsulated inside the xylan-based hydrogel. TMPyP-loaded hydrogel prolonged release of PS up to 24 h with a cumulative amount that could reach 100%. TMPyP-loaded hydrogel showed a photocytotoxic effect against , , strains, and , while no cytotoxicity was observed in the dark.
光动力抗菌化疗或 PACT 已被证明是一种很有前途的抗菌治疗方法，可以克服耐多药细菌的挑战。然而，大多数现有光敏剂的使用受到其显著的自猝灭效应、差的水溶性、对细菌细胞缺乏选择性以及可能对周围组织的损伤的严重阻碍。水凝胶的使用可能克服其中的一些限制。我们在此报告了一种从山毛榉木聚糖合成交联水凝胶的简单策略。该水凝胶具有高达 62 的高溶胀比、相互连接的多孔结构和良好的机械完整性，并且选择 5,10,15,20-四（1-甲基吡啶-4-基）卟啉四碘化物（TMPyP）作为亲水性光敏剂（PS）的模型并将其封装在木聚糖基水凝胶内。载 TMPyP 的水凝胶将 PS 的释放延长至 24 小时，累积量可达 100%。载 TMPyP 的水凝胶对 、 、 菌株和 表现出光细胞毒性作用，而在黑暗中观察不到细胞毒性。
